TY  - JOUR
A1  - Stothut, Manuel
A1  - Kühne, Damaris
A1  - Ströbele, Vanessa
A1  - Mahla, Lisa
A1  - Künzel, Sven
A1  - Krehenwinkel, Henrik
T1  - Environmental DNA metabarcoding reliably recovers arthropod interactions which are frequently observed by video recordings of flowers
T2  - Environmental DNA
N2  - Environmental DNA (eDNA) metabarcoding promises to be a cost- and time-efficient monitoring tool to detect interactions of arthropods with plants. However, observation-based verification of the eDNA-derived data is still required to confirm the reliability of those detections, i.e., to verify whether the arthropods have previously interacted with the plant. Here, we conducted a comparative analysis of the performance of eDNA metabarcoding and video camera observations to detect arthropod communities associated with sunflowers (Helianthus annuus, L.). We compared the taxonomic composition, interaction type, and diversity by testing for an effect of arthropod interaction time and occupancy on successful taxon recovery by eDNA. We also tested if prewashing of the flowers successfully removed eDNA deposition from before the video camera recording, thus enabling a reset of the community for standardized monitoring. We find that eDNA and video camera observations recovered distinct communities, with about a quarter of the arthropod families overlapping. However, the overlapping taxa comprised ~90% of the interactions observed by the video camera. Interestingly, eDNA metabarcoding recovered more unique families than the video cameras, but approximately two-thirds of those unique observations were of rare species. The eDNA-derived families were biased toward plant sap-suckers, showing that such species may deposit more eDNA than, for example, transient pollinators. We also find that prewashing of the flower heads did not suffice to remove all eDNA traces, suggesting that eDNA on plants may be more temporally stable than previously thought. Our work highlights the great potential of eDNA as a tool to detect plant-arthropod interactions, particularly for specialized and frequently interacting taxa.
KW  - biodiversity
KW  - eDNA
KW  - high throughput sequencing
KW  - plant-arthropod interaction
KW  - pollinators
KW  - DNS
KW  - Umwelt
KW  - DNA Barcoding
KW  - Videoüberwachung
KW  - Sonnenblume
KW  - Gliederfüßer
KW  - Biodiversität
Y1  - 2024
UR  - https://ubt.opus.hbz-nrw.de/frontdoor/index/index/docId/2459
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:385-1-24598
VL  - 2024
IS  - 6 / 3
PB  - Wiley
CY  - Hoboken
ER  -