Julian Hans

• Biotic communities experienced significant changes in recent decades. Climate change, the overexploitation of natural resources and the immigration of invasive species are major drivers for this change and present unknown challenges for communities worldwide. To assess the impact of these drivers, standardised long-term studies are required, which are currently lacking for many species and ecosystems. Analysing environmental samples and the DNA of associated organisms using metabarcoding and high-throughput sequencing provides a cost-efficient and rapid way to generate the high-resolution biodiversity data which is so direly needed. In this thesis, I demonstrate the great potential of using samples from the German Environ- mental Specimen Bank (ESB), a long-term monitoring archive that has been collecting and cryogenically storing highly standardised environmental samples since 1985. Modern analytical methods enable retrospective long-term biodiversity monitoring using these samples. In the first chapter, I illustrate metabarcoding as a central method, discussing its strengths and drawbacks, how to avoid them, and new application approaches. This chapter provides the methodological basis for the following studies. In subsequent chapters, I present time series analyses of communities associated with these environmental samples. While for Chapter two the focus is on terrestrial arthropod communities, in Chapter three aquatic and terrestrial communities across the tree of life are analysed. A null model was developed for this survey for robust conclusions. The studies covered the last three decades and revealed substantial compositional changes across all ecosystems. These changes deviated significantly from the model, indicating that the changes are occurring faster than expected. Moreover, a trend toward homogenization in many terrestrial communities was uncovered. Climate change and the immigration of invasive species in combination with the loss of site-specific species are suspected to be the main drivers for this. In a follow-up study, changes of arthropod communities in German and South Korean terrestrial ecosystems were compared using ESB leaf samples from these two countries. Since both ESBs are harmonised in sample collection and processing, comparative analyses were applicable. This research covered the last decade and revealed substantial declines in species richness in Korea. Abiotic and biotic factors are discussed as potential drivers of these results. Finally, the possibility of assessing tree health by analysing changes in functional fungal groups using German ESB samples was investigated. The results indicate that increasing infestation of specific functional groups is a proxy for declining tree health, with further analyses planned. In this dissertation, I present the great potential of samples from long-term monitoring archives to conduct retrospective biodiversity trend analyses across the tree of life. As technologies evolve, these samples will help to understand past and predict future ecosystem changes.