In and with this thematic issue we would like to invite you to engage in productive boundary work and to critically examine the relationship between nature and culture in the Anthropocene. A few years ago, the term Anthropocene was proposed by Paul Crutzen as a term for the current geological epoch, in which humankind (the ‘anthropos’) is seen as the central driving force for global changes in ecological systems. This epoch is characterized by the blurring of boundaries between society and nature, science and politics, as well as by the increased drawing of boundaries between social groups, lifestyles, and the Global North and Global South. With this issue, we would like to give an impetus to explore boundary phenomena in the relationship between nature and society, which up to now have not been the focus of Border Studies. The challenges and problems of the Anthropocene require cross-border thinking and research that stimulates a new reflexivity and commitment, to which the multidisciplinary field of Border Studies can contribute.