Perennial crops eliminate soil disturbance and reduce the amount of synthetic chemicals that are applied to the soil, improving soil biodiversity and food web structure. Additionally, perennial cropping is characterised by all year-round surface coverage which benefits soil biota in terms of habitat and food sources. Perennial intermediate wheatgrass (Thinopyrum intermedium, IWG) was domesticated and commercialised by The Land Institute in Kansas as Kernza® and serves as an example for these nature-based solutions. It develops an extensive root system that has a higher nutrient retention, possibly reducing nutrient runoff. It thereby follows a more resource-conservative strategy with improved belowground-oriented resource allocation in its root system. This may reduce the need for excessive fertiliser as the crop has a higher nitrogen efficiency, among other things. IWG promoted the earthworm community and its diversity, more specifically, the occurrence of epigeic species (litter inhabitants), since those species benefit from the increased soil coverage and elimination of disturbances in the soil. As IWG creates a dense and extensive root system, as shown by the increased occurrence of root-feeding nematodes, endogeic species (horizontal burrowers) are supported through the provision of a reliable food source. IWG was characterised as a mostly undisturbed system with a highly structured food web through nematode analysis, as expressed through the promotion of structure indicators, for example, that are sensitive to disturbances in the soil and are therefore supported under no-till management. The root microbiome is continuously being shaped by the host as the crop regrows from the roots each vegetation period. This creates a symbiotic relationship and a beneficial feedback loop for the crop. Resultantly, the root-endophytic microbiome under IWG had a higher network complexity, connectivity and stability compared to annual wheat. The regrowth from the roots for IWG requires increased nutrient and energy storage, which was indicated by increased starch values. Correspondingly, the longer residence time of the roots in the soil resulted in higher lignin values. Furthermore, the decomposition pathway was dominated by fungivorous nematodes which may correspond to stimulated nutrient cycling and a heterogeneous resource environment, as seen for low input systems. Overall, perennial wheat cultivation improved soil biodiversity already after an establishment of 3-6 years. As those benefits were present for all three countries, the varying soil and climate conditions do not seem to interfere with the positive effect of perennial wheat on the soil ecosystem, demonstrating a wide transferability and adaptability of the crop onto other study sites as well. Enhanced complexity and connectivity of the food web in comparison to annual wheat may indicate a resistance against abiotic stress, suggesting IWG cultivation as a viable option for a sustainable and resilient agriculture. The improvement in nutrient cycling and the resource-efficient cultivation strategy for IWG could enable cultivation on marginal land where annual crop cultivation is not possible as the soils are susceptible to erosion and nutrient runoff. This opens up new possibilities for agricultural cultivation on previously unused land, thus contributing to food security in the future.

Die Verfügbarkeit von ortho-Phosphat (o-PO₄) trägt wesentlich zur Eutrophierung von Fließgewässern bei und gefährdet damit das Erreichen des „guten ökologischen Zustands“ gemäß der EU-Wasserrahmenrichtlinie. Da die kommunalen Kläranlagen zentrale Eintragsquellen darstellen, gewinnt die Reduktion von o-PO₄ an dieser Stelle an Bedeutung. Neben der chemischen Phosphorelimination bietet insbesondere die vierte Reinigungsstufe, primär zur Entfernung von Mikroschadstoffen konzipiert, einen Synergieeffekt mit potenziellen Phosphorentfernungsraten von bis zu 85 %. Zur Bewertung des Einflusses einer solchen Reinigungsstufe wurde ein Modell für ausgewählte saarländische Oberflächenwasserkörper (OWK) entwickelt, das den Trockenwetterfall als eutrophierungsrelevantes Szenario abbildet. Ein zentraler Bestandteil ist ein neu erarbeiteter Retentionsansatz, der biochemische und physikalische Prozesse wie Adsorption, Sedimentation und biologische Assimilation berücksichtigt. Auf Basis der Differenz zwischen emissionsseitig bilanziertem und gemessenem o-PO₄-Gehalt wurden für jeden OWK Verminderungsraten je Fließmeter abgeleitet und schließlich eine Gleichung zur Abschätzung der Retention in Abhängigkeit der Einzugsgebietsgröße formuliert. Die Validierung zeigt hinreichende Modellgenauigkeit, wenngleich negative Frachtdifferenzen in einigen Gewässern auf zusätzliche, nicht eindeutig quantifizierbare Einträge – etwa aus Landwirtschaft oder Kanalverlusten – hindeuten. Die Szenarienanalyse belegt, dass eine vierte Reinigungsstufe grundsätzlich zur Reduktion von o-PO₄ an den Messstellen beiträgt. Eine Unterschreitung des geltenden Orientierungswertes wird jedoch nur erreicht, wenn sämtliche Kläranlagen eines OWK nachgerüstet werden – und auch dann nur in einigen Fällen. Damit stellt die vierte Reinigungsstufe allein keine ausreichende Alternative zu den Maßnahmen des 3. Bewirtschaftungsplans des Saarlandes dar, kann jedoch als ergänzende Strategie zur Verringerung der Phosphoreinträge dienen.

Einige Forschungsergebnisse zeigen, dass emotionale Empfindungen kognitive Bereiche beeinflussen oder mit diesen im Zusammenhang stehen. Aufbauend auf den Ergebnissen wurden zwei Studien konzipiert. In Studie 1 wurde der Zusammenhang zwischen den Valenzen der dispositionalen emotionalen Empfindungen und der globalen Selbstbewertung des Gedächtnisses (Metagedächtnis) bei Lehramtsstudierenden (N = 218) untersucht. Die dispositionalen Empfindungen wurden mittels des deutschen Positive and Negativ Affect Schedule (PANAS) (Krohne, Egloff, Kohlmann & Tausch, 1996) und die globale Selbstbewertung des Gedächtnisses mit dem deutschen Squire Subjective Memory Questionnaire (SSMQ) (Wolf, 2017) erfasst. Angenommen wurde, dass die positive Valenz im Gegensatz zu der negativen Valenz im positiven Zusammenhang mit der höheren Gedächtniseinschätzung stehen. Die Ergebnisse bestätigen die Hypothesen. In Studie 2 wurde die aktuelle Valenz mittels des Open Affective Standardized Image Set (OASIS) (Kurdi, Lozano & Banaji, 2017) induziert, um Veränderungen des Metagedächtnisses und der tatsächlichen Gedächtnisleistung bei Lehramtsstudierenden (N = 44) zu untersuchen. Angenommen wurde, dass die positive Valenz positiv, die negative Valenz negativ und die neutrale Valenz nicht auf das Metagedächtnis und die Gedächtnisleistung wirkt. Weitere Zusammenhänge zwischen dem Metagedächtnis und der Gedächtnisleistung sowie der induzierten Valenz und der Gedächtnisleistung wurden angenommen. Die Messinstrumente aus Studie 1 blieben dieselben. Die Gedächtnisleistung wurde mittels eines sinnarmen Silbentests nach Ebbinghaus (1885) operationalisiert. Die Ergebnisse bestätigen die Hypothesen nicht. Die Emotionsinduktion hatte keinen Erfolg. Die Ergebnisse können damit nicht auf eine veränderte Valenz bezogen werden. Wie in Studie 1 zeigte sich ein Zusammenhang zwischen den dispositionalen Empfindungen und dem Metagedächtnis. Weitere explorative Ergebnisse, vor allem im Bezug auf das Geschlecht, wurden dargestellt. Die Ergebnisse sind bedeutsam für die Professionalisierung von Lehramtsstudierenden.

Biodiversity is threatened by a wide range of anthropogenic activities. Monitoring offers critical insights into how and why biodiversity is changing, helping to identify effective measures for maintaining biodiversity and its ecosystem services. However, conventional biodiversity monitoring methods are labor-intensive, and standardized long-term monitoring series are scarce. DNA-based approaches like metabarcoding environmental DNA (eDNA) promise rapid, cost-efficient, and highly resolved community data. At the same time, scientists are looking for alternative data sources that can compensate for the lack of long-term monitoring data to study past biodiversity changes. This work explores the potential of the German Environmental Specimen Bank (ESB), a pollution monitoring archive, which appears particularly promising for retrospective biodiversity monitoring. Biota samples from different ecosystems across the country are collected and archived at an exceptional level of standardization. Sampling species act as natural eDNA samplers, accumulating genetic traces from surrounding organisms. The cryogenic storage at the ESB preserves any eDNA in the samples in its original state. In this thesis, Chapter I serves as an introductory chapter, outlining the general chances and challenges of metabarcoding for assessing biodiversity. Chapter II focuses on primer design and testing the utility of ESB sampling species like mussels and macroalgae for characterizing the surrounding community. Both chapters form the basis for Chapters III to V, which report the use of ESB time series to uncover sample-associated communities and the changes they undergo. Chapter III illustrates the value of these time series by revealing the invasion trajectory of an alien barnacle into German coastal waters and linking the process to climate change. Chapter IV forms the core of this thesis by presenting an expanded measurement of biodiversity change in ESB time series across different taxonomic groups and ecosystem types. Here, a gradual compositional change (turnover) is reported from bacterial, fungal, microeukaryotic, and metazoan communities tending to either spatial homogenization or differentiation. Observed trends are tested for significance using a dynamic model of community ecology based on the equilibrium theory of island biogeography. The model reveals significantly accelerated turnover rates across all taxonomic groups and ecosystems investigated, suggesting a common, anthropogenically induced driver of biodiversity change. Since these analyses most likely include DNA derived from dead as well as from living organisms, Chapter V aims to separate both groups by metabarcoding both DNA and less stable ribosomal RNA from mussel samples. Contrary to the hypothesis, RNA is detectable from both living endobionts and dietary taxa. However, it outcompetes DNA in detecting microeukaryotic biodiversity. In summary, this thesis demonstrates the outstanding potential of archived ESB samples for retrospective biodiversity monitoring, a resource that offers many further untapped opportunities for future biodiversity research at multiple scales.

Social virtual environments allow their users to meet and collaborate in a shared three-dimensional space, even when far apart from each other in the real world. Within these spaces, the appearance and interaction capabilities of both users and environments can be adapted and changed in a myriad of ways. To enable virtual environments to fulfill their potential of supporting a wide variety of collaboration use-cases, both the impacts of basic interaction design decisions and the individual needs of specific usage areas need to be explored further. This thesis approaches this topic in two ways. First, the basic building blocks of collaboration in social virtual environments are explored by asking the question: "How can social virtual spaces that allow interaction beyond real-world constraints utilize the potential of mutual assistance and shared workflows between multiple users?". Going into further detail for a serious use-case in which direct collaborative interactions and their effect on the included users are especially important, it then explores the potential of collaborative virtual spaces in the therapy domain by asking "How can the potential of social virtual spaces be utilized to support and improve therapy encounters?" With regards to the first research question, the thesis presents two theoretical frameworks detailing different aspects of supporting smooth and varied collaboration processes. In addition, several user studies on the topic of collaborative virtual interaction are described, focusing on the role that different users can play during shared interaction and the effects that this distribution of roles and responsibilities has on both the performance and experience of the involved user pairs. The results presented for this first research question show that social virtual spaces have the potential to provide dedicated support for collaborative workflows. To enable users to adapt their working mode individually and as a team, interaction techniques should complement a team's natural interaction and communication. When presenting novel interactions to users, providing them with a way to support each other can ease their adaptation to these interactions. In these cases, the inclusion of all interested collaborators as active participators should be prioritized in order to let all users benefit from being immersed in a virtual environment. Addressing the combination of social virtual spaces with therapy in relation to the second research question, this thesis presents the result of a series of interviews with practicing physio- and psychotherapists. Motivated by the recorded expert feedback, it also reports on two more detailed explorations of specific areas of interest. The work presented for the second research question demonstrated the promise of using virtual environments in both exercise- and conversation-based therapy practice. Investigating the potential of shared interactions, the exploration of virtual recordings and the adaptation of virtual appearances, the presented work uncovered several topic areas that could be further explored regarding their possible use in the treatment of patients. Taken together, the six research articles presented in this thesis show both the value of supporting and understanding shared interactions in virtual spaces and their potential place in serious use-cases like the therapy domain. When introducing shared virtual environments to new user groups, the opportunity for mutual support through shared interaction techniques could be a crucial building block towards making virtual spaces both accessible and attractive to a variety of users.