Amphibians globally suffer from emerging infectious diseases like chytridiomycosis caused by the continuously spreading chytrid fungi. One is Batrachochytrium salamandrivorans (Bsal) and its disease ‒ the ‘salamander plague’ ‒ which is lethal to several caudate taxa. Recently introduced into Western Europe, long distance dispersal of Bsal, likely through human mediation, has been reported. Herein we study if Alpine salamanders (Salamandra atra and S. lanzai) are yet affected by the salamander plague in the wild. Members of the genus Salamandra are highly susceptible to Bsal leading to the lethal disease. Moreover, ecological modelling has shown that the Alps and Dinarides, where Alpine salamanders occur, are generally suitable for Bsal. We analysed skin swabs of 818 individuals of Alpine salamanders and syntopic amphibians at 40 sites between 2017 to 2022. Further, we compiled those with published data from 319 individuals from 13 sites concluding that Bsal infections were not detected. Our results suggest that the salamander plague so far is absent from the geographic ranges of Alpine salamanders. That means that there is still a chance to timely implement surveillance strategies. Among others, we recommend prevention measures, citizen science approaches, and ex situ conservation breeding of endemic salamandrid lineages.

With the advent of high­throughput sequencing (HTS), profiling immunoglobulin (IG) repertoires has become an essential part of immunological research. The dissection of IG repertoires promises to transform our understanding of the adaptive immune system dynamics. Advances in sequencing technology now also allow the use of the Ion Torrent Personal Genome Machine (PGM) to cover the full length of IG mRNA transcripts. The applications of this bench­top scale HTS platform range from identification of new therapeutic antibodies to the deconvolution of malignant B cell tumors. In the context of this thesis, the usability of the PGM is assessed to investigate the IG heavy chain (IGH) repertoires of animal models. First, an innovate bioinformatics approach is presented to identify antigendriven IGH sequences from bulk sequenced bone marrow samples of transgenic humanized rats, expressing a human IG repertoire (OmniRatTM). We show, that these rats mount a convergent IGH CDR3 response towards measles virus hemagglutinin protein and tetanus toxoid, with high similarity to human counterparts. In the future, databases could contain all IGH CDR3 sequences with known specificity to mine IG repertoire datasets for past antigen exposures, ultimately reconstructing the immunological history of an individual. Second, a unique molecular identifier (UID) based HTS approach and network property analysis is used to characterize the CLL­like CD5+ B cell expansion of A20BKO mice overexpressing a natural short splice variant of the CYLD gene (A20BKOsCYLDBOE). We could determine, that in these mice, overexpression of sCYLD leads to unmutated subvariant of CLL (U­CLL). Furthermore, we found that this short splice variant is also seen in human CLL patients highlighting it as important target for future investigations. Third, the UID based HTS approach is improved by adapting it to the PGM sequencing technology and applying a custom­made data processing pipeline including the ImMunoGeneTics (IMGT) database error detection. Like this, we were able to obtain correct IGH sequences with over 99.5% confidence and correct CDR3 sequences with over 99.9% confidence. Taken together, the results, protocols and sample processing strategies described in this thesis will improve the usability of animal models and the Ion Torrent PGM HTS platform in the field if IG repertoire research.

This dissertation includes three research articles on the portfolio risks of private investors. In the first article, we analyze a large data set of private banking portfolios in Switzerland of a major bank with the unique feature that parts of the portfolios were managed by the bank, and parts were advisory portfolios. To correct the heterogeneity of individual investors, we apply a mixture model and a cluster analysis. Our results suggest that there is indeed a substantial group of advised individual investors that outperform the bank managed portfolios, at least after fees. However, a simple passive strategy that invests in the MSCI World and a risk-free asset significantly outperforms both the better advisory and the bank managed portfolios. The new regulation of the EU for financial products (UCITS IV) prescribes Value at Risk (VaR) as the benchmark for assessing the risk of structured products. The second article discusses the limitations of this approach and shows that, in theory, the expected return of structured products can be unbounded while the VaR requirement for the lowest risk class can still be satisfied. Real-life examples of large returns within the lowest risk class are then provided. The results demonstrate that the new regulation could lead to new seemingly safe products that hide large risks. Behavioral investors who choose products based only on their official risk classes and their expected returns will, therefore, invest into suboptimal products. To overcome these limitations, we suggest a new risk-return measure for financial products based on the martingale measure that could erase such loopholes. Under the mean-VaR framework, the third article discusses the impacts of the underlying's first four moments on the structured product. By expanding the expected return and the VaR of a structured product with its underlying moments, it is possible to investigate each moment's impact on them, simultaneously. Results are tested by Monte Carlo simulation and historical simulation. The findings show that for the majority of structured products, underlyings with large positive skewness are preferred. The preferences for variance and for kurtosis are ambiguous.

This thesis considers the general task of computing a partition of a set of given objects such that each set of the partition has a cardinality of at least a fixed number k. Among such kinds of partitions, which we call k-clusters, the objective is to find the k-cluster which minimises a certain cost derived from a given pairwise difference between objects which end up the same set. As a first step, this thesis introduces a general problem, denoted by (||.||,f)-k-cluster, which models the task to find a k-cluster of minimum cost given by an objective function computed with respect to specific choices for the cost functions f and ||.||. In particular this thesis considers three different choices for f and also three different choices for ||.|| which results in a total of nine different variants of the general problem. Especially with the idea to use the concept of parameterised approximation, we first investigate the role of the lower bound on the cluster cardinalities and find that k is not a suitable parameter, due to remaining NP-hardness even for the restriction to the constant 3. The reductions presented to show this hardness yield the even stronger result which states that polynomial time approximations with some constant performance ratio for any of the nine variants of (||.||,f)-k-cluster require a restriction to instances for which the pairwise distance on the objects satisfies the triangle inequality. For this restriction to what we informally refer to as metric instances, constant-factor approximation algorithms for eight of the nine variants of (||.||,f)-k-cluster are presented. While two of these algorithms yield the provably best approximation ratio (assuming P!=NP), others can only guarantee a performance which depends on the lower bound k. With the positive effect of the triangle inequality and applications to facility location in mind, we discuss the further restriction to the setting where the given objects are points in the Euclidean metric space. Considering the effect of computational hardness caused by high dimensionality of the input for other related problems (curse of dimensionality) we check if this is also the source of intractability for (||.||,f)-k-cluster. Remaining NP-hardness for restriction to small constant dimensionality however disproves this theory. We then use parameterisation to develop approximation algorithms for (||.||,f)-k-cluster without restriction to metric instances. In particular, we discuss structural parameters which reflect how much the given input differs from a metric. This idea results in parameterised approximation algorithms with parameters such as the number of conflicts (our name for pairs of objects for which the triangle inequality is violated) or the number of conflict vertices (objects involved in a conflict). The performance ratios of these parameterised approximations are in most cases identical to those of the approximations for metric instances. This shows that for most variants of (||.||,f)-k-cluster efficient and reasonable solutions are also possible for non-metric instances.

This doctoral thesis includes five studies that deal with the topics work, well-being, and family formation, as well as their interaction. The studies aim to find answers to the following questions: Do workers’ personality traits determine whether they sort into jobs with performance appraisals? Does job insecurity result in lower quality and quantity of sleep? Do public smoking bans affect subjective well-being by changing individuals’ use of leisure time? Can risk preferences help to explain non-traditional family forms? And finally, are differences in out-of-partnership birth rates between East and West Germany driven by cultural characteristics that have evolved in the two separate politico-economic systems? To answer these questions, the following chapters use basic economic subjects such as working conditions, income, and time use, but also employ a range of sociological and psychological concepts such as personality traits and satisfaction measures. Furthermore, all five studies use data from the German Socio-Economic Panel (SOEP), a representative longitudinal panel of private households in Germany, and apply state-of-the-art microeconometric methods. The findings of this doctoral thesis are important for individuals, employers, and policymakers. Workers and employers benefit from knowing the determinants of occupational sorting, as vacancies can be filled more accurately. Moreover, knowing which job-related problems lead to lower well-being and potentially higher sickness absence likely increases efficiency in the workplace. The research on smoking bans and family formation in chapters 4, 5, and 6 is particularly interesting for policymakers. The results on the effects of smoking bans on subjective well-being presented in chapter 4 suggest that the impacts of tobacco control policies could be weighed more carefully. Additionally, understanding why women are willing to take the risks associated with single motherhood can help to improve policies targeting single mothers.