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Background
The morphology of anuran larvae is suggested to differ between species with tadpoles living in standing (lentic) and running (lotic) waters. To explore which character combinations within the general tadpole morphospace are associated with these habitats, we studied categorical and metric larval data of 123 (one third of which from lotic environments) Madagascan anurans.
Results
Using univariate and multivariate statistics, we found that certain combinations of fin height, body musculature and eye size prevail either in larvae from lentic or lotic environments.
Conclusion
Evidence for adaptation to lotic conditions in larvae of Madagascan anurans is presented. While lentic tadpoles typically show narrow to moderate oral discs, small to medium sized eyes, convex or moderately low fins and non-robust tail muscles, tadpoles from lotic environments typically show moderate to broad oral discs, medium to big sized eyes, low fins and a robust tail muscle.
Objective: Attunement is a novel measure of nonverbal synchrony reflecting the duration of the present moment shared by two interaction partners. This study examined its association with early change in outpatient psychotherapy.
Methods: Automated video analysis based on motion energy analysis (MEA) and cross-correlation of the movement time-series of patient and therapist was conducted to calculate movement synchrony for N = 161 outpatients. Movement-based attunement was defined as the range of connected time lags with significant synchrony. Latent change classes in the HSCL-11 were identified with growth mixture modeling (GMM) and predicted by pre-treatment covariates and attunement using multilevel multinomial regression.
Results: GMM identified four latent classes: high impairment, no change (Class 1); high impairment, early response (Class 2); moderate impairment (Class 3); and low impairment (Class 4). Class 2 showed the strongest attunement, the largest early response, and the best outcome. Stronger attunement was associated with a higher likelihood of membership in Class 2 (b = 0.313, p = .007), Class 3 (b = 0.251, p = .033), and Class 4 (b = 0.275, p = .043) compared to Class 1. For highly impaired patients, the probability of no early change (Class 1) decreased and the probability of early response (Class 2) increased as a function of attunement.
Conclusions: Among patients with high impairment, stronger patient-therapist attunement was associated with early response, which predicted a better treatment outcome. Video-based assessment of attunement might provide new information for therapists not available from self-report questionnaires and support therapists in their clinical decision-making.
In recent decades, the Arctic has been undergoing a wide range of fast environmental changes. The sea ice covering the Arctic Ocean not only reacts rapidly to these changes, but also influences and alters the physical properties of the atmospheric boundary layer and the underlying ocean on various scales. In that regard, polynyas, i.e. regions of open water and thin ice within thernclosed pack ice, play a key role as being regions of enhanced atmosphere-ice-ocean interactions and extensive new ice formation during winter. A precise long-term monitoring and increased efforts to employ long-term and high-resolution satellite data is therefore of high interest for the polar scientific community. The retrieval of thin-ice thickness (TIT) fields from thermal infrared satellite data and atmospheric reanalysis, utilizing a one-dimensional energy balance model, allows for the estimation of the heat loss to the atmosphere and hence, ice-production rates. However, an extended application of this approach is inherently connected with severe challenges that originate predominantly from the disturbing influence of clouds and necessary simplifications in the model set-up, which all need to be carefully considered and compensated for. The presented thesis addresses these challenges and demonstrates the applicability of thermal infrared TIT distributions for a long-term polynya monitoring, as well as an accurate estimation of ice production in Arctic polynyas at a relatively high spatial resolution. Being written in a cumulative style, the thesis is subdivided into three parts that show the consequent evolution and improvement of the TIT retrieval, based on two regional studies (Storfjorden and North Water (NOW) polynya) and a final large-scale, pan-Arctic study. The first study on the Storfjorden polynya, situated in the Svalbard archipelago, represents the first long-term investigation on spatial and temporal polynya characteristics that is solely based on daily TIT fields derived from MODIS thermal infrared satellite data and ECMWF ERA-Interim atmospheric reanalysis data. Typical quantities such as polynya area (POLA), the TIT distribution, frequencies of polynya events as well as the total ice production are derived and compared to previous remote sensing and modeling studies. The study includes a first basic approach that aims for a compensation of cloud-induced gaps in daily TIT composites. This coverage-correction (CC) is a mathematically simple upscaling procedure that depends solely on the daily percentage of available MODIS coverage and yields daily POLA with an error-margin of 5 to 6 %. The NOW polynya in northern Baffin Bay is the main focus region of the second study, which follows two main goals. First, a new statistics-based cloud interpolation scheme (Spatial Feature Reconstruction - SFR) as well as additional cloud-screening procedures are successfully adapted and implemented in the TIT retrieval for usage in Arctic polynya regions. For a 13-yr period, results on polynya characteristics are compared to the CC approach. Furthermore, an investigation on highly variable ice-bridge dynamics in Nares Strait is presented. Second, an analysis of decadal changes of the NOW polynya is carried out, as the additional use of a suite of passive microwave sensors leads to an extended record of 37 consecutive winter seasons, thereby enabling detailed inter-sensor comparisons. In the final study, the SFR-interpolated daily TIT composites are used to infer spatial and temporal characteristics of 17 circumpolar polynya regions in the Arctic for 2002/2003 to 2014/2015. All polynya regions combined cover an average thin-ice area of 226.6 -± 36.1 x 10-³ km-² during winter (November to March) and yield an average total wintertime accumulated ice production of about 1811 -± 293 km-³. Regional differences in derived ice production trends are noticeable. The Laptev Sea on the Siberian shelf is presented as a focus region, as frequently appearing polynyas along the fast-ice edge promote high rates of new ice production. New affirming results on a distinct relation to sea-ice area export rates and hence, the Transpolar Drift, are shown. This new high-resolution pan-Arctic data set can be further utilized and build upon in a variety of atmospheric and oceanographic applications, while still offering room for further improvements such as incorporating high-resolution atmospheric data sets and an optimized lead-detection.
Optimal control problems are optimization problems governed by ordinary or partial differential equations (PDEs). A general formulation is given byrn \min_{(y,u)} J(y,u) with subject to e(y,u)=0, assuming that e_y^{-1} exists and consists of the three main elements: 1. The cost functional J that models the purpose of the control on the system. 2. The definition of a control function u that represents the influence of the environment of the systems. 3. The set of differential equations e(y,u) modeling the controlled system, represented by the state function y:=y(u) which depends on u. These kind of problems are well investigated and arise in many fields of application, for example robot control, control of biological processes, test drive simulation and shape and topology optimization. In this thesis, an academic model problem of the form \min_{(y,u)} J(y,u):=\min_{(y,u)}\frac{1}{2}\|y-y_d\|^2_{L^2(\Omega)}+\frac{\alpha}{2}\|u\|^2_{L^2(\Omega)} subject to -\div(A\grad y)+cy=f+u in \Omega, y=0 on \partial\Omega and u\in U_{ad} is considered. The objective is tracking type with a given target function y_d and a regularization term with parameter \alpha. The control function u takes effect on the whole domain \Omega. The underlying partial differential equation is assumed to be uniformly elliptic. This problem belongs to the class of linear-quadratic elliptic control problems with distributed control. The existence and uniqueness of an optimal solution for problems of this type is well-known and in a first step, following the paradigm 'first optimize, then discretize', the necessary and sufficient optimality conditions are derived by means of the adjoint equation which ends in a characterization of the optimal solution in form of an optimality system. In a second step, the occurring differential operators are approximated by finite differences and the hence resulting discretized optimality system is solved with a collective smoothing multigrid method (CSMG). In general, there are several optimization methods for solving the optimal control problem: an application of the implicit function theorem leads to so-called black-box approaches where the PDE-constrained optimization problem is transformed into an unconstrained optimization problem and the reduced gradient for these reduced functional is computed via the adjoint approach. Another possibilities are Quasi-Newton methods, which approximate the Hessian by a low-rank update based on gradient evaluations, Krylov-Newton methods or (reduced) SQP methods. The use of multigrid methods for optimization purposes is motivated by its optimal computational complexity, i.e. the number of required computer iterations scales linearly with the number of unknowns and the rate of convergence, which is independent of the grid size. Originally multigrid methods are a class of algorithms for solving linear systems arising from the discretization of partial differential equations. The main part of this thesis is devoted to the investigation of the implementability and the efficiency of the CSMG on commodity graphics cards. GPUs (graphic processing units) are designed for highly parallelizable graphics computations and possess many cores of SIMD-architecture, which are able to outperform the CPU regarding to computational power and memory bandwidth. Here they are considered as prototype for prospective multi-core computers with several hundred of cores. When using GPUs as streamprocessors, two major problems arise: data have to be transferred from the CPU main memory to the GPU main memory, which can be quite slow and the limited size of the GPU main memory. Furthermore, only when the streamprocessors are fully used to capacity, a remarkable speed-up comparing to a CPU is achieved. Therefore, new algorithms for the solution of optimal control problems are designed in this thesis. To this end, a nonoverlapping domain decomposition method is introduced which allows the exploitation of the computational power of many GPUs resp. CPUs in parallel. This algorithm is based on preliminary work for elliptic problems and enhanced for the application to optimal control problems. For the domain decomposition into two subdomains the linear system for the unknowns on the interface is solved with a Schur complement method by using a discrete approximation of the Steklov-Poincare operator. For the academic optimal control problem, the arising capacitance matrix can be inverted analytically. On this basis, two different algorithms for the nonoverlapping domain decomposition for the case of many subdomains are proposed in this thesis: on the one hand, a recursive approach and on the other hand a simultaneous approach. Numerical test compare the performance of the CSMG for the one domain case and the two approaches for the multi-domain case on a GPU and CPU for different variants.
Background
In light of the current biodiversity crisis, DNA barcoding is developing into an essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often rely on short amplicon sequences, which yield accurate identification of biological entities in a community but provide limited phylogenetic resolution across broad taxonomic scales. However, the phylogenetic structure of communities is an essential component of biodiversity. Consequently, a barcoding approach is required that unites robust taxonomic assignment power and high phylogenetic utility. A possible solution is offered by sequencing long ribosomal DNA (rDNA) amplicons on the MinION platform (Oxford Nanopore Technologies).
Findings
Using a dataset of various animal and plant species, with a focus on arthropods, we assemble a pipeline for long rDNA barcode analysis and introduce a new software (MiniBar) to demultiplex dual indexed Nanopore reads. We find excellent phylogenetic and taxonomic resolution offered by long rDNA sequences across broad taxonomic scales. We highlight the simplicity of our approach by field barcoding with a miniaturized, mobile laboratory in a remote rainforest. We also test the utility of long rDNA amplicons for analysis of community diversity through metabarcoding and find that they recover highly skewed diversity estimates.
Conclusions
Sequencing dual indexed, long rDNA amplicons on the MinION platform is a straightforward, cost-effective, portable, and universal approach for eukaryote DNA barcoding. Although bulk community analyses using long-amplicon approaches may introduce biases, the long rDNA amplicons approach signifies a powerful tool for enabling the accurate recovery of taxonomic and phylogenetic diversity across biological communities.
Mental processes are filters which intervene in the literary presentation of nature. This article will take you on a journey through literary landscapes, starting from Joseph Furphy and end-ing with Gerald Murnane. It will try to show the development of Australian literary landscape depiction. The investigation of this extensive topic will show that the perception of the Aus-tralian landscape as foreign and threatening is a coded expression of the protagonists" crisis of identity due to their estrangement from European cultural roots. Only a feeling of being at home enables the characters to perceive landscapes in a positive way and allows the author to depict intimate and familiar views of nature. This topic will be investigated with a range of novels to reveal the development of this theme from the turn of the nineteenth century (the time of Furphy- novel Such is Life) up to the present (i.e. novels by Malouf, Foster, Hall, Murnane).
1.The Discursive Construction of Black Masculinity: Intersections of Race, Gender, and Sexuality
1.1.The Plight of Black Men: A History of Lynchings and Castrations
1.2.The Discursive Construction of the Black Man as Otherrn
1.3.Black Corporeality and the Scopic Regime of Racism
2. Ralph Ellison's 'Invisible man'
2.1.Invisible Black Men: Between Emasculation and Hypermasculinityrn
2.2.Transcending Invisibility
During the last decade, anatomic and physiological neuroscience research has yielded extensive information on the physiological regulators of short-term satiety, visceral and interoceptive sensation. Distinct neural circuits regulate the elements of food ingestion physiologically. The general aim of the current studies is to elucidate the peripheral neural pathways to the brain in healthy subjects to establish the groundwork for the study of the pathophysiology of bulimia nervosa (BN). We aimed to define the central activation pattern during non-nutritive gastric distension in humans, and aimed to define the cognitive responses to this mechanical gastric distension. We estimated regional cerebral blood flow with 15O-water positron emission tomography during intragastric balloon inflation and deflation in 18 healthy young women of normal weight. The contrast between inflated minus deflated in the exploratory analysis revealed activation in more than 20 brain regions. The analysis confirmed several well known areas in the central nervous system that contribute to visceral processing: the inferior frontal cortex, representing a zone of convergence for food related stimuli; the insula and operculum referred to as "visceral cortex"; the anterior cingulate gyrus (and insula), processing affective information; and the brainstem, a site of vagal relay for visceral afferent stimuli. Brain activation in the left ventrolateral prefrontal cortex was reproducible. This area is well known for higher cognitive processing, especially reward-related stimuli. The ventrolateral prefrontal cortex with the insular regions may provide a link between the affective and rewarding components of eating and disordered eating as observed in BN and binge-eating obesity. Gastric distension caused a significant rapid, reversible, and reproducible increase in the feelings of fullness, sleepiness, and gastric discomfort as well as a significant rapid, reversible, and reproducible decrease in the feeling of hunger. We showed that mechanical activation of the neurocircuitry involved in meal termination led to the described phenomena. The current brain activation studies of non-painful, proximal gastric distension could provide groundwork in the field of abnormal eating behavior by suggesting a link between visceral sensation and abnormal eating patterns. A potential treatment for disordered eating and obesity could alter the conscious and unconscious perception and interoceptive awareness of gastric distension contributing to meal termination.
The aim of the thesis was to investigate the role of the immune system in fibromyalgia (FM), as part of a dynamic co-regulation between different bodily systems. FM is a chronic musculoskeletal disorder characterized by widespread pain and specific tender points, combined with other symptoms including fatigue, sleep disturbances, morning stiffness and anxiety. The main goal of the work was to identify possible dysregulation of peripheral immune and endocrine parameters in patients with FM compared to matched healthy controls. Moreover, the possible relation between symptom complaints and the specific parameters measured was also evaluated. A first approach was to investigate possible differences between FM patients and controls in the expression of cytokines, as they have been implicated in the occurrence of several of the symptoms associated with FM. Furthermore, adhesion molecules which are involved in cell-to-cell communication and immune cell trafficking were also studied. The latter are known to be regulated by both cytokines and glucocorticoids (GCs) and their expression is often found altered in patients with immune dysregulation. It was expected that subjects with FM would have an increased production of proinflammatory cytokines and/or a reduced antiinflammatory cytokine production and that certain cytokines and/or adhesion molecules would be differently regulated by dexamethasone (DEX). Unstimulated blood was used in the analysis of adhesion molecule expression by flow cytometry while stimulated whole blood cell cultures were used in cytokine flow cytometry assays. Peripheral blood mononuclear cells (PBMCs) were also cultured and the supernatants collected to determine the concentration of cytokines by biochip protein array. In addition, serum samples were used in enzyme-linked immunosorbent assays (ELISA) for quantification of soluble adhesion molecules. L-selectin was found elevated on monocytes and neutrophils of FM patients. A bias toward lower IL-4 levels was observed in FM patients. Based on studies showing differences in glucocorticoid receptor (GR) affinity and disturbances associated with loss of hypothalamic-pituitary-adrenal (HPA) axis resiliency in FM, it was hypothesized whether FM would be associated with abnormalities in glucocorticoid sensitivity. Total plasma cortisol and salivary free cortisol were quantified by ELISA and time-resolved fluorescence immunoassay, respectively. GR sensitivity through DEX inhibition of IL-6, in stimulated whole blood, was evaluated after cytokine quantification by ELISA. The corticosteroid receptors, GR alpha and mineralocorticoid receptor (MR), as well as the glucocorticoid-induced leucine zipper (GILZ) and the FK506 binding protein 5 mRNA expression were assessed in PBMCs by real-time reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, sequencing of RT-PCR products and/or genomic DNA was used for mutational analysis of the corticosteroid receptors. We observed lower basal plasma cortisol levels (borderline statistical significance) and a lower expression of corticosteroid receptors and GILZ in FM patients when compared to healthy controls. The minor allele of the MR single nucleotide polymorphism (SNP) rs5522 was found more often in FM patients than in controls. In addition, female carriers of this SNP seemed to have reduced salivary cortisol responses to a strong psychological stressor (Trier Social Stress Test) compared to non-carriers. FM patient carriers of an MR intronic SNP (rs17484245), before exon 3, were associated with significantly higher scores of depression symptoms compared to patient non-carriers. The thesis includes also a comprehensive analysis of the complexity of GR regulation and the role of alternative mRNA splicing. It focuses on the differential expression of the untranslated GR first exons, their high sequence homology among different species and how genetic determinants, without apparent relevance, may have implications in health and disease. In FM patients, GR exon 1-C expression was found lower and a significant difference was observed when comparing GR 1-C expression between antidepressant-free and patients who had taken antidepressants until two weeks before sample collection. In summary, the study shows a slight disturbance of some components of the innate immune system of FM patients and suggests an enhanced adhesion and possible recruitment of leukocytes to inflammatory sites. The reduced expression of corticosteroid receptors and possibly the reduced MR function may be associated with an impaired function of the HPA axis in these patients. A hyporesponsiveness of the HPA axis under stress or disturbances of the stress response could make these patients more vulnerable to cytokines and inflammation which, compounded by lower antiinflammatory mediators, may sustain some of the symptoms that contribute to the clinical picture of FM.
Background and rationale: Changing working conditions demand adaptation, resulting in higher stress levels in employees. In consequence, decreased productivity, increasing rates of sick leave, and cases of early retirement result in higher direct, indirect, and intangible costs. Aims of the Research Project: The aim of the study was to test the usefulness of a novel translational diagnostic tool, Neuropattern, for early detection, prevention, and personalized treatment of stress-related disorders. The trial was designed as a pilot study with a wait list control group. Materials and Methods: In this study, 70 employees of the Forestry Department Rhineland-Palatinate, Germany, were enrolled. Subjects were block-randomized according to the functional group of their career field, and either underwent Neuropattern diagnostics immediately, or after a waiting period of three months. After the diagnostic assessment, their physicians received the Neuropattern Medical Report, including the diagnostic results and treatment recommendations. Participants were informed by the Neuropattern Patient Report, and were eligible to an individualized Neuropattern Online Counseling account. Results: The application of Neuropattern diagnostics significantly improved mental health and health-related behavior, reduced perceived stress, emotional exhaustion, overcommitment and possibly, presenteeism. Additionally, Neuropattern sensitively detected functional changes in stress physiology at an early stage, thus allowing timely personalized interventions to prevent and treat stress pathology. Conclusion: The present study encouraged the application of Neuropattern diagnostics to early intervention in non-clinical populations. However, further research is required to determine the best operating conditions.