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This thesis deals with economic aspects of employees' sickness. In addition to the classical case of sickness absence, in which an employee is completely unable to work and hence stays at home, there is the case of sickness presenteeism, in which the employee comes to work despite being sick. Accordingly, the thesis at hand covers research on both sickness states, absence and presenteeism. The first section covers sickness absence and labour market institutions. Chapter 2 presents theoretical and empirical evidence that differences in the social norm against benefit fraud, so-called benefit morale, can explain cross country diversity in the generosity of statutory sick pay entitlements between developed countries. In our political economy model, a stricter benefit morale reduces the absence rate, with counteracting effects on the politically set sick pay replacement rate. On the one hand, less absence caused by a stricter norm, makes the tax-financed insurance cheaper, leading to the usual demand side effect and hence to more generous sick pay entitlements. On the other hand, being less likely to be absent due to a stricter norm, the voters prefer a smaller fee over more insurance. We document both effects in a sample of 31 developed countries, capturing the years from 1981 to 2010. In Chapter 3 we investigate the relationship between the existence of works councils and illness-related absence and its consequences for plants. Using individual data from the German Socio-Economic Panel (SOEP), we find that the existence of a works council is positively correlated with the incidence and the annual duration of absence. Additionally, linked employer-employee data (LIAB) suggests that employers are more likely to expect personnel problems due to absence in plants with a works council. In western Germany, we find significant effects using a difference-in-differences approach, which can be causally interpreted. The second part of this thesis covers two studies on sickness presenteeism. In Chapter 4, we empirically investigate the determinants of the annual duration of sickness presenteeism using the European Working Conditions Survey (EWCS). Work autonomy, workload and tenure are positively related to the number of sickness presenteeism days, while a good working environment comes with less presenteeism. In Chapter 5 we theoretically and empirically analyze sickness absence and presenteeism behaviour with a focus on their interdependence. Specifically, we ask whether work-related factors lead to a substitutive, a complementary or no relationship between sickness absence and presenteeism. In other words, we want to know whether changes in absence and presenteeism behaviour incurred by work-related characteristics point in opposite directions (substitutive), the same direction (complementary), or whether they only affect either one of the two sickness states (no relationship). Our theoretical model shows that the relationship between sickness absence and presenteeism with regard to work-related characteristics is not necessarily of a substitutive nature. Instead, a complementary or no relationship can emerge as well. Turning to the empirical investigation, we find that only one out of 16 work-related factors, namely the supervisor status, leads to a substitutive relationship between absence and presenteeism. Few of the other determinants are complements, while the large majority is either related to sickness absence or presenteeism.
A basic assumption of standard small area models is that the statistic of interest can be modelled through a linear mixed model with common model parameters for all areas in the study. The model can then be used to stabilize estimation. In some applications, however, there may be different subgroups of areas, with specific relationships between the response variable and auxiliary information. In this case, using a distinct model for each subgroup would be more appropriate than employing one model for all observations. If no suitable natural clustering variable exists, finite mixture regression models may represent a solution that „lets the data decide“ how to partition areas into subgroups. In this framework, a set of two or more different models is specified, and the estimation of subgroup-specific model parameters is performed simultaneously to estimating subgroup identity, or the probability of subgroup identity, for each area. Finite mixture models thus offer a fexible approach to accounting for unobserved heterogeneity. Therefore, in this thesis, finite mixtures of small area models are proposed to account for the existence of latent subgroups of areas in small area estimation. More specifically, it is assumed that the statistic of interest is appropriately modelled by a mixture of K linear mixed models. Both mixtures of standard unit-level and standard area-level models are considered as special cases. The estimation of mixing proportions, area-specific probabilities of subgroup identity and the K sets of model parameters via the EM algorithm for mixtures of mixed models is described. Eventually, a finite mixture small area estimator is formulated as a weighted mean of predictions from model 1 to K, with weights given by the area-specific probabilities of subgroup identity.
Major threats to the Spanish Constitutional Court’s independence and authority have come, first, from political parties and the media and, second, by the Catalonian secession movement. The authority and the legitimacy of the Constitutional Court were tested in the stormy
proceedings on the Statute of Autonomy of Catalonia of 2006 that ended in 2010 and, above all, in the period of 2013–2017, when successive acts directed at the secession of were recurrently Catalonia challenged before the Court and subsequently overturned, and to stop the continued disobedience its rulings the of Court was given extended execution powers for its judgments. These new powers include the temporary replacement of any authority or public official that does not comply with a Court’s ruling and the ordering of a substitutive execution through the central government. The Court declared the new powers to be consistent with the Constitution (with three dissenting votes by four constitutional judges) and it even used them for the first time to enforce its prohibition of the referendum on the independence of Catalonia of 1 October 2017. Nevertheless, the Venice Commission has raised doubts about the opportunity of those powers, which are unusual in European constitutional jurisdiction models. At the end, the Court’s powers were not enough to stop the Catalonian secession process, and on 27 October 2017 the state government implemented the federal coercion clause and suspended Catalonian autonomy until new elections were held.
The Second Language Acquisition of English Non-Finite Complement Clauses – A Usage-Based Perspective
(2022)
One of the most essential hypotheses of usage-based theories and many constructionist approaches to language is that language entails the piecemeal learning of constructions on the basis of general cognitive mechanisms and exposure to the target language in use (Ellis 2002; Tomasello 2003). However, there is still a considerable lack of empirical research on the emergence and mental representation of constructions in second language (L2) acquisition. One crucial question that arises, for instance, is whether L2 learners’ knowledge of a construction corresponds to a native-like mapping of form and meaning and, if so, to what extent this representation is shaped by usage. For instance, it is unclear how learners ‘build’ constructional knowledge, i.e. which pieces of frequency-, form- and meaning-related information become relevant for the entrenchment and schematisation of a L2 construction.
To address these issues, the English catenative verb construction was used as a testbed phenomenon. This idiosyncratic complex construction is comprised of a catenative verb and a non-finite complement clause (see Huddleston & Pullum 2002), which is prototypically a gerund-participial (henceforth referred to as ‘target-ing’ construction) or a to-infinitival complement (‘target-to’ construction):
(1) She refused to do her homework.
(2) Laura kept reading love stories.
(3) *He avoids to listen to loud music.
This construction is particularly interesting because learners often show choices of a complement type different from those of native speakers (e.g. Gries & Wulff 2009; Martinez‐Garcia & Wulff 2012) as illustrated in (3) and is commonly claimed to be difficult to be taught by explicit rules (see e.g. Petrovitz 2001).
By triangulating different types of usage data (corpus and elicited production data) and analysing these by multivariate statistical tests, the effects of different usage-related factors (e.g. frequency, proficiency level of the learner, semantic class of verb, etc.) on the representation and development of the catenative verb construction and its subschemas (i.e. target-to and target-ing construction) were examined. In particular, it was assessed whether they can predict a native-like form-meaning pairing of a catenative verb and non-finite complement.
First, all studies were able to show a robust effect of frequency on the complement choice. Frequency does not only lead to the entrenchment of high-frequency exemplars of the construction but is also found to motivate a taxonomic generalisation across related exemplars and the representation of a more abstract schema. Second, the results indicate that the target-to construction, due to its higher type and token frequency, has a high degree of schematicity and productivity than the target-ing construction for the learners, which allows for analogical comparisons and pattern extension with less entrenched exemplars. This schema is likely to be overgeneralised to (less frequent) target-ing verbs because the learners perceive formal and semantic compatibility between the unknown/infrequent verb and this pattern.
Furthermore, the findings present evidence that less advanced learners (A2-B2) make more coarse-grained generalisations, which are centred around high-frequency and prototypical exemplars/low-scope patterns. In the case of high-proficiency learners (C1-C2), not only does the number of native-like complement choices increase but relational information, such as the semantic subclasses of the verb, form-function contingency and other factors, becomes also relevant for a target-like choice. Thus, the results suggests that with increasing usage experience learners gradually develop a more fine-grained, interconnected representation of the catenative verb construction, which gains more resemblance to the form-meaning mappings of native speakers.
Taken together, these insights highlight the importance for language learning and teaching environments to acknowledge that L2 knowledge is represented in the form of highly interconnected form-meaning pairings, i.e. constructions, that can be found on different levels of abstraction and complexity.
Up until May 2021, the post-election insecurity in Belarus had mostly been a national affair, but with Lukashenka’s regime starting to retaliate against foreign actors, the crisis internationalised. This article follows the development of Belarus-Lithuania border dynamics between the 2020 Belarusian presidential election and the start of the 2022 Russian invasion of Ukraine. A qualitative content analysis of English-language articles published by Lithuanian public broadcaster LRT shows that shows that there were relatively few changes to the border dynamics in the period between 9 August 2020 and 26 May 2021. After 26 May 2021, the border dynamics changed significantly: The Belarusian regime started facilitating migration, and more than 4,200 irregular migrants crossed into Lithuania from Belarus in 2021. In response, Lithuania reinforced its border protection and tried to deal with the irregular migration flows. Calls for action were made, protests were held, and the country received international support.
The Constitution of Latvia
(2004)
The article offers a concise view on the constitution of the Baltic state of Latvia. After an introduction focusing on constitutional history, the author explores basic principles and human rights in the text of the constitution and explains the main constitutional bodies and their functions in legislative, executive and judiciary. Chapters on citizenship and religious rights round up this introduction to the Latvian Constitution.
ENGLISH ACADEMIC LITERARY DISCOURSE IN SOUTH AFRICA 1958-2004: A REVIEW OF 11 ACADEMIC JOURNALS
(2007)
This study examines the discipline of English studies in South Africa through a review of articles published in 11 academic journals over the period 1958"2004. The aims are to gain a better understanding of the functions of peer-reviewed journals, to reveal the presence of rules governing discursive production, and to uncover the historical shifts in approach and choice of disciplinary objects. The Foucauldian typology of procedures determining discursive production, that is: exclusionary, internal and restrictive procedures, is applied to the discipline of English studies in order to elucidate the existence of such procedures in the discipline. Each journal is reviewed individually and comparatively. Static and chronological statistical analyses are undertaken on the articles in the 11 journals in order to provide empirical evidence to subvert the contention that the discipline is unruly and its choice of objects random. The cumulative results of this analysis are used to describe the major shifts primarily in ranges of disciplinary objects, but also in metadiscursive and thematic debates. Each of the journals is characterised in relation to what the overall analysis reveals about the mainstream developments. The two main findings are that, during the period under review, South African imaginative written artefacts have moved from a marginal position to the centre of focus of the discipline; and that the conception of what constitutes the "literary" has returned to a pre-Practical criticism definition, broadly inclusive of a variety of types of artefact including imaginative writing, such as autobiography, letters, journals and orature.
This working paper outlines analytical pathways that could contribute to deepening the understanding of water inequalities in cities of the Global South. It brings together the status quo of research on water inequalities in Accra, the capital of Ghana, and studies on Environmental Justice. In doing so, it argues for the need to analytically distinguish between the terms ‘(in)equality’ and ‘(in)justice’. Studying everyday water practices and per- spectives on water (in)justice of different stakeholders would be a suitable entry point for an in-depth ethnographic study that analytically separates water inequalities and water injustices but considers their interlinkages. The working paper is based on a literature review conducted in 2015 in the scope of the WaterPower project.
This work is concerned with the numerical solution of optimization problems that arise in the context of ground water modeling. Both ground water hydraulic and quality management problems are considered. The considered problems are discretized problems of optimal control that are governed by discretized partial differential equations. Aspects of special interest in this work are inaccurate function evaluations and the ensuing numerical treatment within an optimization algorithm. Methods for noisy functions are appropriate for the considered practical application. Also, block preconditioners are constructed and analyzed that exploit the structure of the underlying linear system. Specifically, KKT systems are considered, and the preconditioners are tested for use within Krylov subspace methods. The project was financed by the foundation Stiftung Rheinland-Pfalz für Innovation and carried out in joint work with TGU GmbH, a company of consulting engineers for ground water and water resources.
Behavioural traces from interactions with digital technologies are diverse and abundant. Yet, their capacity for theory-driven research is still to be constituted. In the present cumulative dissertation project, I deliberate the caveats and potentials of digital behavioural trace data in behavioural and social science research. One use case is online radicalisation research. The three studies included, set out to discern the state-of-the-art of methods and constructs employed in radicalization research, at the intersection of traditional methods and digital behavioural trace data. Firstly, I display, based on a systematic literature review of empirical work, the prevalence of digital behavioural trace data across different research strands and discern determinants and outcomes of radicalisation constructs. Secondly, I extract, based on this literature review, hypotheses and constructs and integrate them to a framework from network theory. This graph of hypotheses, in turn, makes the relative importance of theoretical considerations explicit. One implication of visualising the assumptions in the field is to systematise bottlenecks for the analysis of digital behavioural trace data and to provide the grounds for the genesis of new hypotheses. Thirdly, I provide a proof-of-concept for incorporating a theoretical framework from conspiracy theory research (as a specific form of radicalisation) and digital behavioural traces. I argue for marrying theoretical assumptions derived from temporal signals of posting behaviour and semantic meaning from textual content that rests on a framework from evolutionary psychology. In the light of these findings, I conclude by discussing important potential biases at different stages in the research cycle and practical implications.
Evidence points to autonomy as having a place next to affiliation, achievement, and power as one of the basic implicit motives; however, there is still some research that needs to be conducted to support this notion.
The research in this dissertation aimed to address this issue. I have specifically focused on two issues that help solidify the foundation of work that has already been conducted on the implicit autonomy motive, and will also be a foundation for future studies. The first issue is measurement. Implicit motives should be measured using causally valid instruments (McClelland, 1980). The second issue addresses the function of motives. Implicit motives orient, select, and energize behavior (McClelland, 1980). If autonomy is an implicit motive, then we need a valid instrument to measure it and we also need to show that it orients, selects, and energizes behavior.
In the following dissertation, I address these two issues in a series of ten studies. Firstly, I present studies that examine the causal validity of the Operant Motive Test (OMT; Kuhl, 2013) for the implicit affiliation and power motives using established methods. Secondly, I developed and empirically tested pictures to specifically assess the implicit autonomy motive and examined their causal validity. Thereafter, I present two studies that investigated the orienting and energizing effects of the implicit autonomy motive. The results of the studies solidified the foundation of the OMT and how it measures nAutonomy. Furthermore, this dissertation demonstrates that nAutonomy fulfills the criteria for two of the main functions of implicit motives. Taken together, the findings of this dissertation provide further support for autonomy as an implicit motive and a foundation for intriguing future studies.
When do anorexic patients perceive their body as too fat? Aggravating and ameliorating factors
(2019)
Objective
Our study investigated body image representations in female patients with anorexia nervosa
and healthy controls using a size estimation with pictures of their own body. We also
explored a method to reduce body image distortions through right hemispheric activation.
Method
Pictures of participants’ own bodies were shown on the left or right visual fields for 130 ms
after presentation of neutral, positive, or negative word primes, which could be self-relevant
or not, with the task of classifying the picture as “thinner than”, “equal to”, or “fatter than”
one’s own body. Subsequently, activation of the left- or right hemispheric through right- or
left-hand muscle contractions for 3 min., respectively. Finally, participants completed the
size estimation task again.
Results
The distorted “fatter than” body image was found only in patients and only when a picture of
their own body appeared on the right visual field (left hemisphere) and was preceded by
negative self-relevant words. This distorted perception of the patients’ body image was
reduced after left-hand muscle contractions (right hemispheric activation).
Discussion
To reduce body image distortions it is advisable to find methods that help anorexia nervosa
patients to increase their self-esteem. The body image distortions were ameliorated after
right hemispheric activation. A related method to prevent distorted body-image representations
in these patients may be Eye Movement Desensitization and Reprocessing (EMDR)
therapy.
Background: The body-oriented therapeutic approach Somatic Experiencing® (SE) treats posttraumatic symptoms by changing the interoceptive and proprioceptive sensations associated with the traumatic experience. Filling a gap in the landscape of trauma treatments, SE has attracted growing interest in research and therapeutic practice, recently.
Objective: To date, there is no literature review of the effectiveness and key factors of SE. This review aims to summarize initial findings on the effectiveness of SE and to outline methodspecific key factors of SE.
Method: To gain a first overview of the literature, we conducted a scoping review including studies until 13 August 2020. We identified 83 articles of which 16 fit inclusion criteria and were systematically analysed.
Results: Findings provide preliminary evidence for positive effects of SE on PTSD-related symptoms. Moreover, initial evidence suggests that SE has a positive impact on affective and somatic symptoms and measures of well-being in both traumatized and non-traumatized
samples. Practitioners and clients identified resource-orientation and use of touch as methodspecific key factors of SE. Yet, an overall studies quality assessment as well as a Cochrane analysis of risk of bias indicate that the overall study quality is mixed.
Conclusions: The results concerning effectiveness and method-specific key factors of SE are promising; yet, require more support from unbiased RCT-research. Future research should focus on filling this gap.
Striving for sustainable development by combating climate change and creating a more social world is one of the most pressing issues of our time. Growing legal requirements and customer expectations require also Mittelstand firms to address sustainability issues such as climate change. This dissertation contributes to a better understanding of sustainability in the Mittelstand context by examining different Mittelstand actors and the three dimensions of sustainability - social, economic, and environmental sustainability - in four quantitative studies. The first two studies focus on the social relevance and economic performance of hidden champions, a niche market leading subgroup of Mittelstand firms. At the regional level, the impact of 1,645 hidden champions located in Germany on various dimensions of regional development is examined. A higher concentration of hidden champions has a positive effect on regional employment, median income, and patents. At the firm level, analyses of a panel dataset of 4,677 German manufacturing firms, including 617 hidden champions, show that the latter have a higher return on assets than other Mittelstand firms. The following two chapters deal with environmental strategies and thus contribute to the exploration of the environmental dimension of sustainability. First, the consideration of climate aspects in investment decisions is compared using survey data from 468 European venture capital and private equity investors. While private equity firms respond to external stakeholders and portfolio performance and pursue an active ownership strategy, venture capital firms are motivated by product differentiation and make impact investments. Finally, based on survey data from 443 medium-sized manufacturing firms in Germany, 54% of which are family-owned, the impact of stakeholder pressures on their decarbonization strategies is analyzed. A distinction is made between symbolic (compensation of CO₂-emissions) and substantive decarbonization strategies (reduction of CO₂-emissions). Stakeholder pressures lead to a proactive pursuit of decarbonization strategies, with internal and external stakeholders varying in their influence on symbolic and substantial decarbonization strategies, and the relationship influenced by family ownership.
This study focuses on the representation of British South Asian identities in contemporary British audiovisual media. It attempts to answer the question, whether these identities are represented as hybrid, heterogeneous and ambivalent, or whether these contemporary representations follow in the tradition of colonial and postcolonial racialism. Racialised depictions of British South Asians have been the norm not only in the colonial but also in the postcolonial era until the rise of the Black British movement, whose successes have been also acknowledged in the field of representation. However these achievements have to be scrutinized again, especially in the context of the post 9/11 world, rising Islamophobia, and new forms of institutionalized discrimination on the basis of religion. Since the majority of British Muslims are of South Asian origin, this study tries to answer the question whether the marker of religious origin is racial belonging, i.e. skin colour, and old stereotypes associated with the racialised representation are being perpetuated into current depictions through an examination of the varied genre of popular audio visual media texts.
Evapotranspiration (ET) is one of the most important variables in hydrological studies. In the ET process, energy exchange and water transfer are involved. ET consists of transpiration and evaporation. The amount of plants transpiration dominates in ET. Especially in the forest regions, the ratio of transpiration to ET is in general 80-90 %. Meteorological variables, vegetation properties, precipitation and soil moisture are critical influence factors for ET generation. The study area is located in the forest area of Nahe catchment (Rhineland-Palatinate, Germany). The Nahe catchment is highly wooded. About 54.6 % of this area is covered by forest, with deciduous forest and coniferous forest are two primary types. A hydrological model, WaSiM-ETH, was employed for a long-term simulation from 1971-2003 in the Nahe catchment. In WaSiM-ETH, the potential evapotranspiration (ETP) was firstly calculated by the Penman-Monteith equation, and subsequently reduced according to the soil water content to obtain the actual evapotranspiration (ETA). The Penman-Monteith equation has been widely used and recommended for ETP estimation. The difficulties in applying this equation are the high demand of ground-measured meteorological data and the determination of surface resistance. A method combined remote sensing images with ground-measured meteorological data was also used to retrieve the ETA. This method is based on the surface properties such as surface albedo, fractional vegetation cover (FVC) and land surface temperature (LST) to obtain the latent heat flux (LE, corresponding to ETA) through the surface energy balance equation. LST is a critical variable for surface energy components estimation. It was retrieved from the TM/ETM+ thermal infrared (TIR) band. Due to the high-quality and cloudy-free requirements for TM/ETM+ data selection as well as the overlapping cycle of TM/ETM+ sensor is 16 days, images on only five dates are available during 1971-2003 (model ran) " May 15, 2000, July 05, 2001, July 19, August 04 and September 21 in 2003. It is found that the climate conditions of 2000, 2001 and 2003 are wet, medium wet and dry, respectively. Therefore, the remote sensing-retrieved observations are noncontinuous in a limited number over time but contain multiple climate conditions. Aerodynamic resistance and surface resistance are two most important parameters in the Penman-Monteith equation. However, for forest area, the aerodynamic resistance is calculated by a function of wind speed in the model. Since transpiration and evaporation are separately calculated by the Penman-Monteith equation in the model, the surface resistance was divided into canopy surface resistance rsc and soil surface resistance rse. rsc is related to the plants transpiration and rse is related to the bare soil evaporation. The interception evaporation was not taken into account due to its negligible contribution to ET rate under a dry-canopy (no rainfall) condition. Based on the remote sensing-retrieved observations, rsc and rse were calibrated in the WaSiM-ETH model for both forest types: for deciduous forest, rsc = 150 sm−1, rse = 250 sm−1; for coniferous forest, rsc = 300 sm−1, rse = 650 sm−1. We also carried out sensitivity analysis on rsc and rse. The appropriate value ranges of rsc and rse were determined as (annual maximum): for deciduous forest, [100,225] sm−1 for rsc and [50,450] sm−1 for rse; for coniferous forest, [225,375] sm−1 for rsc and [350,1200] sm−1 for rse. Due to the features of the observations that are in a limited number but contain multiple climate conditions, the statistical indices for model performance evaluation are required to be sensitive to extreme values. In this study, boxplots were found to well exhibit the model performance at both spatial and temporal scale. Nush-Sutcliffe efficiency (NSE), RMSE-observations standard deviation ratio (RSR), percent bias (PBIAS), mean bias error (MBE), mean variance of error distribution (S2d), index of agreement (d), root mean square error (RMSE) were found as appropriate statistical indices to provide additional evaluation information to the boxplots. The model performance can be judged as satisfactory if NSE > 0.5, RSR ≤ 0.7, PBIAS < -±12, MBE < -±0.45, S2d < 1.11, d > 0.79, RMSE < 0.97. rsc played a more important role than rse in ETP and ETA estimation by the Penman-Monteith equation, which is attributed to the fact that transpiration dominates in ET. The ETP estimation was found the most correlated to the relative humidity (RH), followed by air temperature (T), relative sunshine duration (SSD) and wind speed (WS). Under wet or medium wet climate conditions, ETA estimation was found the most correlated to T, followed by RH, SSD and WS. Under a water-stress condition, there were very small correlations between ETA and each meteorological variable.
The glucocorticoid (GC) cortisol, main mediator of the hypothalamic-pituitary-adrenal axis, has many implications in metabolism, stress response and the immune system. GC function is mediated mainly via the glucocorticoid receptor (GR) which binds as a transcription factor to glucocorticoid response elements (GREs). GCs are strong immunosuppressants and used to treat inflammatory and autoimmune diseases. Long-term usage can lead to several irreversible side effects which make improved understanding indispensable and warrant the adaptation of current drugs. Several large scale gene expression studies have been performed to gain insight into GC signalling. Nevertheless, studies at the proteomic level have not yet been made. The effects of cortisol on monocytes and macrophages were studied in the THP-1 cell line using 2D fluorescence difference gel electrophoresis (2D DIGE) combined with MALDI-TOF mass spectrometry. More than 50 cortisol-modulated proteins were identified which belonged to five functional groups: cytoskeleton, chaperones, immune response, metabolism, and transcription/translation. Multiple GREs were found in the promoters of their corresponding genes (+10 kb/-0.2 kb promoter regions including all alternative promoters available within the Database for Transcription Start Sites (DBTSS)). High quality GREs were observed mainly in cortisol modulated genes, corroborating the proteomics results. Differential regulation of selected immune response related proteins were confirmed by qPCR and immuno-blotting. All immune response related proteins (MX1, IFIT3, SYWC, STAT3, PMSE2, PRS7) which were induced by LPS were suppressed by cortisol and belong mainly to classical interferon target genes. Mx1 has been selected for detailed expression analysis since new isoforms have been identified by proteomics. FKBP51, known to be induced by cortisol, was identified as the strongest differentially expressed protein and contained the highest number of strict GREs. Genomic analysis of five alternative FKBP5 promoter regions suggested GC inducibility of all transcripts. 2D DIGE combined with 2D immunoblotting revealed the existence of several previously unknown FKBP51 isoforms, possibly resulting from these transcripts. Additionally multiple post-translational modifications were found, which could lead to different subcellular localization in monocytes and macrophages as seen by confocal microscopy. Similar results were obtained for the different cellular subsets of human peripheral blood mononuclear cells (PBMCs). FKBP51 was found to be constitutively phosphorylated with up to 8 phosphosites in CD19+ B lymphocytes. Differential Co-immunoprecipitation for cytoplasm and nucleus allowed us to identify new potential interaction partners. Nuclear FKBP51 was found to interact with myosin 9, whereas cytosolic FKBP51 with TRIM21 (synonym: Ro52, Sjögren`s syndrome antigen). The GR has been found to interact with THOC4 and YB1, two proteins implicated in mRNA processing and transcriptional regulation. We also applied proteomics to study rapid non-genomic effects of acute stress in a rat model. The nuclear proteome of the thymus was investigated after 15 min restraint stress and compared to the non-stressed control. Most of the identified proteins were transcriptional regulators found to be enriched in the nucleus probably to assist gene expression in an appropriate manner. The proteomic approach allowed us to further understand the cortisol mediated response in monocytes/macrophages. We identified several new target proteins, but we also found new protein variants and post-translational modifications which need further investigation. Detailed study of FKBP51 and GR indicated a complex regulation network which opened a new field of research. We identified new variants of the anti-viral response protein MX1, displaying differential expression and phosphorylation in the cellular compartments. Further, proteomics allowed us to follow the very early effects of acute stress, which happen prior to gene expression. The nuclear thymocyte proteome of restraint stressed rats revealed an active preparation for subsequent gene expression. Proteomics was successfully applied to study differential protein expression, to identify new protein variants and phosphorylation events as well as to follow translocation. New aspects for future research in the field of cortisol-mediated immune modulation have been added.
This work addresses the algorithmic tractability of hard combinatorial problems. Basically, we are considering \NP-hard problems. For those problems we can not find a polynomial time algorithm. Several algorithmic approaches already exist which deal with this dilemma. Among them we find (randomized) approximation algorithms and heuristics. Even though in practice they often work in reasonable time they usually do not return an optimal solution. If we constrain optimality then there are only two methods which suffice for this purpose: exponential time algorithms and parameterized algorithms. In the first approach we seek to design algorithms consuming exponentially many steps who are more clever than some trivial algorithm (who simply enumerates all solution candidates). Typically, the naive enumerative approach yields an algorithm with run time $\Oh^*(2^n)$. So, the general task is to construct algorithms obeying a run time of the form $\Oh^*(c^n)$ where $c<2$. The second approach considers an additional parameter $k$ besides the input size $n$. This parameter should provide more information about the problem and cover a typical characteristic. The standard parameterization is to see $k$ as an upper (lower, resp.) bound on the solution size in case of a minimization (maximization, resp.) problem. Then a parameterized algorithm should solve the problem in time $f(k)\cdot n^\beta$ where $\beta$ is a constant and $f$ is independent of $n$. In principle this method aims to restrict the combinatorial difficulty of the problem to the parameter $k$ (if possible). The basic hypothesis is that $k$ is small with respect to the overall input size. In both fields a frequent standard technique is the design of branching algorithms. These algorithms solve the problem by traversing the solution space in a clever way. They frequently select an entity of the input and create two new subproblems, one where this entity is considered as part of the future solution and another one where it is excluded from it. Then in both cases by fixing this entity possibly other entities will be fixed. If so then the traversed number of possible solution is smaller than the whole solution space. The visited solutions can be arranged like a search tree. To estimate the run time of such algorithms there is need for a method to obtain tight upper bounds on the size of the search trees. In the field of exponential time algorithms a powerful technique called Measure&Conquer has been developed for this purpose. It has been applied successfully to many problems, especially to problems where other algorithmic attacks could not break the trivial run time upper bound. On the other hand in the field of parameterized algorithms Measure&Conquer is almost not known. This piece of work will present examples where this technique can be used in this field. It also will point out what differences have to be made in order to successfully apply the technique. Further, exponential time algorithms for hard problems where Measure&Conquer is applied are presented. Another aspect is that a formalization (and generalization) of the notion of a search tree is given. It is shown that for certain problems such a formalization is extremely useful.
Attitudes are "the most distinctive and indispensable concept in contemporary social psychology" (Allport, 1935, p. 798). This outstanding position of the attitude concept in social cognitive research is not only reflected in the innumerous studies focusing on this concept but also in the huge number of theoretical approaches that have been put forth since then. Yet, it is still an open question, what attitudes actually are. That is, the question of how attitude objects are represented in memory cannot be unequivocally answered until now (e.g., Barsalou, 1999; Gawronski, 2007; Pratkanis, 1989, Chapter 4). In particular, researchers strongly differ with respect to their assumptions on the content, format and structural nature of attitude representations (Ferguson & Fukukura, 2012). This prevailing uncertainty on what actually constitutes our likes and dislikes is strongly dovetailed with the question of which processes result in the formation of these representations. In recent years, this issue has mainly been addressed in evaluative conditioning research (EC). In a standard EC-paradigm a neutral stimulus (conditioned stimulus, CS) is repeatedly paired with an affective stimulus (unconditioned stimulus, US). The pairing of stimuli then typically results in changes in the evaluation of the CS corresponding to the evaluative response of the US (De Houwer, Baeyens, & Field, 2005). This experimental approach on the formation of attitudes has primarily been concerned with the question of how the representations underlying our attitudes are formed. However, which processes operate on the formation of such an attitude representation is not yet understood (Jones, Olson, & Fazio, 2010; Walther, Nagengast, & Trasselli, 2005). Indeed, there are several ideas on how CS-US pairs might be encoded in memory. Notwithstanding the importance of these theoretical ideas, looking at the existing empirical work within the research area of EC (for reviews see Hofmann, De Houwer, Perugini, Baeyens, & Crombez, 2010; De Houwer, Thomas, & Baeyens, 2001) leaves one with the impression that scientists have skipped the basic processes. Basic processes hereby especially refer to the attentional processes being involved in the encoding of CSs and USs as well as the relation between them. Against the background of this huge gap in current research on attitude formation, the focus of this thesis will be to highlight the contribution of selective attention processes to a better understanding of the representation underlying our likes and dislikes. In particular, the present thesis considers the role of selective attention processes for the solution of the representation issue from three different perspectives. Before illustrating these different perspectives, Chapter 1 is meant to envision the omnipresence of the representation problem in current theoretical as well as empirical work on evaluative conditioning. Likewise, it emphasizes the critical role of selective attention processes for the representation question in classical conditioning and how this knowledge might be used to put forth the uniqueness of evaluative conditioning as compared to classical conditioning. Chapter 2 then considers the differential influence of attentional resources and goal-directed attention on attitude learning. The primary objective of the presented experiment was thereby to investigate whether attentional resources and goal-directed attention exert their influence on EC via changes in the encoding of CS-US relations in memory (i.e., contingency memory). Taking the findings from this experiment into account, Chapter 3 focuses on the selective processing of the US relative to the CS. In particular, the two experiments presented in this chapter were meant to explore the moderating influence of the selective processing of the US in its relation to the CS on EC. In Chapter 4 the important role of the encoding of the US in relation to the CS, as outlined in Chapter 3, is illuminated in the context of different retrieval processes. Against the background of the findings from the two presented experiments, the interplay between the encoding of CS-US contingencies and the moderation of EC via different retrieval processes will be discussed. Finally, a general discussion of the findings, their theoretical implications and future research lines will be outlined in Chapter 5.
The present thesis is devoted to a construction which defies generalisations about the prototypical English noun phrase (NP) to such an extent that it has been termed the Big Mess Construction (Berman 1974). As illustrated by the examples in (1) and (2), the NPs under study involve premodifying adjective phrases (APs) which precede the determiner (always realised in the form of the indefinite article a(n)) rather than following it.
(1) NoS had not been hijacked – that was too strong a word. (BNC: CHU 1766)
(2) He was prepared for a battle if the porter turned out to be as difficult a customer as his wife. (BNC: CJX 1755)
Previous research on the construction is largely limited to contributions from the realms of theoretical syntax and a number of cursory accounts in reference grammars. No comprehensive investigation of its realisations and uses has as yet been conducted. My thesis fills this gap by means of an exhaustive analysis of the construction on the basis of authentic language data retrieved from the British National Corpus (BNC). The corpus-based approach allows me to examine not only the possible but also the most typical uses of the construction. Moreover, while previous work has almost exclusively focused on the formal realisations of the construction, I investigate both its forms and functions.
It is demonstrated that, while the construction is remarkably flexible as concerns its possible realisations, its use is governed by probabilistic constraints. For example, some items occur much more frequently inside the degree item slot than others (as, too and so stand out for their particularly high frequency). Contrary to what is assumed in most previous descriptions, the slot is not restricted in its realisation to a fixed number of items. Rather than representing a specialised structure, the construction is furthermore shown to be distributed over a wide range of possible text types and syntactic functions. On the other hand, it is found to be much less typical of spontaneous conversation than of written language; Big Mess NPs further display a strong preference for the function of subject complement. Investigations of the internal structural complexity of the construction indicate that its obligatory components can optionally be enriched by a remarkably wide range of optional (if infrequent) elements. In an additional analysis of the realisations of the obligatory but lexically variable slots (head noun and head of AP), the construction is highlighted to represent a productive pattern. With the help of the methods of Collexeme Analysis (Stefanowitsch and Gries 2003) and Co-varying Collexeme Analysis (Gries and Stefanowitsch 2004b, Stefanowitsch and Gries 2005), the two slots are, however, revealed to be strongly associated with general nouns and ‘evaluative’ and ‘dimension’ adjectives, respectively. On the basis of an inspection of the most typical adjective-noun combinations, I identify the prototypical semantics of the Big Mess Construction.
The analyses of the constructional functions centre on two distinct functional areas. First, I investigate Bolinger’s (1972) hypothesis that the construction fulfils functions in line with the Principle of Rhythmic Alternation (e.g. Selkirk 1984: 11, Schlüter 2005). It is established that rhythmic preferences co-determine the use of the construction to some extent, but that they clearly do not suffice to explain the phenomenon under study. In a next step, the discourse-pragmatic functions of the construction are scrutinised. Big Mess NPs are demonstrated to perform distinct information-structural functions in that the non-canonical position of the AP serves to highlight focal information (compare De Mönnink 2000: 134-35). Additionally, the construction is shown to place emphasis on acts of evaluation. I conclude the construction to represent a contrastive focus construction.
My investigations of the formal and functional characteristics of Big Mess NPs each include analyses which compare individual versions of the construction to one another (e.g. the As Big a Mess, Too Big a Mess and So Big a Mess Constructions). It is revealed that the versions are united by a shared core of properties while differing from one another at more abstract levels of description. The question of the status of the constructional versions as separate constructions further receives special emphasis as part of a discussion in which I integrate my results into the framework of usage-based Construction Grammar (e.g. Goldberg 1995, 2006).