Extracellular enzymes in microbial communities play a central role in nutrient cycling and the degradation of (pollutant) substances in various natural and anthropogenic systems. Bound in aquatic biofilms, sludge aggregates, or even unbound at their interfaces, they are of great importance for both the environment and human health. In particular, in wastewater treatment plants and inland waters, hydrolytic activities influence the wide-reaching efficiency of nutrient removal and self-purification, thus contributing significantly to overall water quality. The main goal of this dissertation project was to investigate the factors that influence enzymatic activity and the health of microbial communities in activated sludge and river systems, particularly in relation to anthropogenic influences and natural environmental conditions. The aim was to contribute to a better understanding of the sensitivity of our freshwater ecosystems and to support the long-term preservation of water quality and ecological stability. The development and optimization of appropriate methods, as well as their testing and applicability, were the focal points. For this purpose, a fluorometric microplate assay was developed and adapted to determine both extracellular enzyme activities (EEAs) in activated sludge samples and in intact biofilms. Its suitability for field studies was subsequently tested. Inhibition and activity of selected hydrolases under different conditions were investigated to better understand the mechanisms and potential environmental risks posed by anthropogenic influences and seasonal fluctuations of hydrochemical and climatic parameters. The first phase of the doctoral thesis involved studies on the inhibition of alkaline phosphatase in activated sludge by oxyanions. Using the fluorometric microplate assay, the inhibitory effect was sensitively detected over a pH range of 7.0 to 8.5. IC50- and IC20-concentrations were calculated from modeled dose-response functions. It was found that vanadate and tungstate caused strong inhibitory effects, while molybdate moderately inhibited the enzyme. An increasing pH led to a reduction in the inhibitory effect of tungstate and molybdate. The inhibition effects of vanadate were not significantly affected by the pH. In municipal wastewater, the concentrations of such metal ions are usually low, but industrial wastewater may have pollutant loads that can significantly impact the removal of phosphorus-containing compounds, and thus the efficiency of treatment plants. In the second phase, an attempt was made to further adapt the developed methodology to investigate EEA and kinetics in intact freshwater biofilms. Four different types of bead materials (lava, glass, sintered quartz, and ceramics) fitting into a 96-well microplate were tested as carriers for biofilms on both the laboratory and field scale. The analysis included a total of seven hydrolases as representatives of key nutrient cycles such as phosphorus, carbon, and nitrogen: phosphatases, glucosidases, peptidases (two different types), and sulfatase. Experiments with increasing substrate concentrations led to classical kinetic profiles according to the Michaelis-Menten mechanism. This allowed for the prediction of the biofilm enzymes’ response to different substrate concentrations. Parameters such as Vmax and Km could be derived from the modeled curves. Ceramic beads are particularly suitable for long-term studies due to their high stability, while sintered quartz beads should be preferred for the use in stagnant media (material loss under turbulent conditions). Lava and glass beads, on the other and, proved suboptimal for uniform biofilm development due to their surface properties. The potential use of this fast and sensitive test for ecotoxicological or even human-toxicological studies was demonstrated by the effects of caffeine on the activity of PDE. The result of this part of the research represents a powerful tool for assessing environmental pollution and monitoring water quality. The high application potential was clearly highlighted in the final phase of the project. The goal here was to deepen the understanding of interactions between seasonal factors, anthropogenic influences, and biofilm processes in rivers by investigating EEA and biofilm parameters such as biomass and relating them to hydrochemical and climatic factors. Ceramic beads were exposed both upstream and downstream of a wastewater treatment plant discharge and sampled over a period of seven months. EEAs and biomass varied depending on the season and location, with higher microbial activity observed upstream in winter. Winter conditions led to the dilution of most nutrients as well as in an increse of dissolved oxygen. Nutrient concentrations analyzed downstream were significantly higher in the summer. Accumulation of nutrient or pollutants during the summer months cannot be excluded, which may have led to a general reduction in enzyme activities. Potential causes could be inhibitory effects on the enzymes, or a reduced enzyme activity due to a sufficiently high nutrient supply. In general, the sampling site upstream showed a more pronounced seasonal dynamics, with a significant proportion of the variance in biological parameters (activity and biomass) attributable to seasonal factors. A secondary component, likely reflecting the impact of the treatment plant discharge, explained another portion of the data variance. Regardless of the season, high correlations between biological parameters were observed upstream, while downstream the data were more decorrelated. This could be because the biofilms, under chronic stress, respond less dynamically to seasonal fluctuations. This dissertation illustrates that in addition to anthropogenic stress factors, seasonal fluctuations of hydrochemical and climatic parameters should also be considered in "stress downstream the pipe" studies. The selected methods are recommended for explaining and considering the data variance, as they highlight the complex interplay between microbial enzymatic activity, environmental factors, and pollutants in the activated sludge of wastewater treatment plants and also in aquatic systems. The novel bead assay could pave the way for the future standardization of effect-oriented studies on intact aquatic biofilms.

The application of machine learning and deep learning methods to hydrological modelling has advanced significantly in recent years, offering alternatives to traditional conceptual and physically based approaches. Within the numerous algorithms, long short-term memory (LSTM) networks have proven themselves particularly useful for the task of streamflow modelling. This thesis provides a collection of publications that investigate the capabilities, limitations and interpretability of LSTM for the purpose of streamflow modelling and climate change impact assessment within the lowland Ems catchment in Northwest Germany. Within a comparative performance evaluation, LSTM and its predecessor, the recurrent neural network, demonstrate superior accuracy compared to the conceptual HBV model across various statistical performance metrics. However, a decline in performance was observed during low-flow conditions in certain sub-catchments. The evaluation of the flow duration curve revealed that the ML models more effectively capture the water balance, while HBV better represents streamflow dynamics. To enhance the interpretability of LSTM, six explainable artificial intelligence techniques were applied. These methods consistently identified seasonal patterns in the temporal relevance of hydroclimatic input data. In combination with an observed correlation between the internal LSTM states and catchment-scale soil moisture dynamics, the findings suggest that LSTM models are capable of implicitly learning the relevant hydrological processes. Following, the capabilities of LSTM to model climate change impact scenarios, particularly when they extend beyond historically observed climate conditions, are addressed. An ensemble of climate change projections is provided as hydroclimatic input to evaluate the performance of LSTMs and conceptual models. While all models reveal heterogeneous alterations in streamflow under future climate conditions, significant differences emerge based on the model type. Results provide evidence that LSTMs, in combination with the temperature-based Haude formula for estimating potential evaporation, work inadequately under altered climatic regimes, raising concerns about their applicability in long-term projections. The study also indicates the potential need to incorporate physical constraints into LSTM architectures to ensure model robustness and hydrological plausibility beyond the historical training range. Collectively, this thesis contributes important insights into the applicability and interpretability of LSTM models in streamflow modelling. Despite the presence of a physically realistic representation of soil moisture dynamics of the Ems catchment, no robust change signals for streamflow under climate change can be derived. Those results underscore the potential of LSTM model approaches for accurate streamflow simulation, however, they require us to always critically question LSTM results, particularly when they are applied outside the training range.

Present-day air quality is known through dense monitoring and extensive pollu- tion control mechanisms. In contrast, knowledge of historical pollution, particularly before the industrial revolution, is accessible only through occasional reports of singular local events and through natural archives such as ice or sediment cores that record global-scale pollution. However, the regular local to regional pollution that most affects human life is hardly known. Historical sciences have argued both for and against significant air pollution in and around historic cities and manufacturing sites. For the Roman era, it has been hypothesized that air quality played a role in several patterns of action of the period. However, to the author's knowledge, there are no quantitative studies of Roman emissions. Using the results of modern experimental archaeology, this study attempts to quantify the emissions from Roman pottery kilns and their impact on surrounding human settlements. It is shown that although the pollution did not reach today's limits, it must have approached levels known to cause adverse health effects. A series of additional test simulations have been conducted to determine how these first results might be improved in the future.

Nel libro Dèi e Zangrèi il professor Pasquale Casile scandaglia con mirabile precisione scientifica e in tutta profondità gli abissi della memoria linguistica dei Greci di Calabria. Fornisce risposte valide ai quesiti: Chi sono gli Zangrèi? Sono gli ultimi Dionisiaci della storia e gli eredi diretti delle comunità orfico-pitagoriche della Magna Grecia? E perché vengono così chiamati anche i Catari di Sicilia?

The dissertation is devoted to the construction and justification of three-point difference schemes of high order of accuracy for solving the Sturm-Liouville problem. A new algorithmic realization of the exact three-point difference scheme on a non-uniform grid has been developed. We show that to compute the coefficients of the exact scheme in an arbitrary grid node, it is necessary to solve two auxiliary Cauchy problems for the system of three linear ordinary differential equations of the first order. The coefficient stability of the exact three-point difference scheme is proved. If the Cauchy problems are solved numerically using any one-step method, we obtain the truncated three-point difference scheme. The accuracy estimate of three-point difference schemes was obtained and the algorithm for finding their solution was developed. We also developed a new algorithmic realization of the exact three-point difference scheme for the Sturm-Liouville problem with singularities at the ends of the interval. As in the case of the classical Sturm-Liouville problem, to find the coefficients of the exact three-point difference scheme, it is necessary to solve two auxiliary Cauchy problems for each grid node. The coefficient stability of the exact three-point difference scheme is proved. Since the Cauchy problems for the first and last grid nodes are singular, the Taylor series method has been developed to solve them. The accuracy estimate of truncated three-point difference schemes was obtained. To solve the difference scheme, the Newton's iterative method is used. Numerical experiments are presented which confirm the efficiency of the proposed approach.

Biodiversity is threatened by a wide range of anthropogenic activities. Monitoring offers critical insights into how and why biodiversity is changing, helping to identify effective measures for maintaining biodiversity and its ecosystem services. However, conventional biodiversity monitoring methods are labor-intensive, and standardized long-term monitoring series are scarce. DNA-based approaches like metabarcoding environmental DNA (eDNA) promise rapid, cost-efficient, and highly resolved community data. At the same time, scientists are looking for alternative data sources that can compensate for the lack of long-term monitoring data to study past biodiversity changes. This work explores the potential of the German Environmental Specimen Bank (ESB), a pollution monitoring archive, which appears particularly promising for retrospective biodiversity monitoring. Biota samples from different ecosystems across the country are collected and archived at an exceptional level of standardization. Sampling species act as natural eDNA samplers, accumulating genetic traces from surrounding organisms. The cryogenic storage at the ESB preserves any eDNA in the samples in its original state. In this thesis, Chapter I serves as an introductory chapter, outlining the general chances and challenges of metabarcoding for assessing biodiversity. Chapter II focuses on primer design and testing the utility of ESB sampling species like mussels and macroalgae for characterizing the surrounding community. Both chapters form the basis for Chapters III to V, which report the use of ESB time series to uncover sample-associated communities and the changes they undergo. Chapter III illustrates the value of these time series by revealing the invasion trajectory of an alien barnacle into German coastal waters and linking the process to climate change. Chapter IV forms the core of this thesis by presenting an expanded measurement of biodiversity change in ESB time series across different taxonomic groups and ecosystem types. Here, a gradual compositional change (turnover) is reported from bacterial, fungal, microeukaryotic, and metazoan communities tending to either spatial homogenization or differentiation. Observed trends are tested for significance using a dynamic model of community ecology based on the equilibrium theory of island biogeography. The model reveals significantly accelerated turnover rates across all taxonomic groups and ecosystems investigated, suggesting a common, anthropogenically induced driver of biodiversity change. Since these analyses most likely include DNA derived from dead as well as from living organisms, Chapter V aims to separate both groups by metabarcoding both DNA and less stable ribosomal RNA from mussel samples. Contrary to the hypothesis, RNA is detectable from both living endobionts and dietary taxa. However, it outcompetes DNA in detecting microeukaryotic biodiversity. In summary, this thesis demonstrates the outstanding potential of archived ESB samples for retrospective biodiversity monitoring, a resource that offers many further untapped opportunities for future biodiversity research at multiple scales.

Social virtual environments allow their users to meet and collaborate in a shared three-dimensional space, even when far apart from each other in the real world. Within these spaces, the appearance and interaction capabilities of both users and environments can be adapted and changed in a myriad of ways. To enable virtual environments to fulfill their potential of supporting a wide variety of collaboration use-cases, both the impacts of basic interaction design decisions and the individual needs of specific usage areas need to be explored further. This thesis approaches this topic in two ways. First, the basic building blocks of collaboration in social virtual environments are explored by asking the question: "How can social virtual spaces that allow interaction beyond real-world constraints utilize the potential of mutual assistance and shared workflows between multiple users?". Going into further detail for a serious use-case in which direct collaborative interactions and their effect on the included users are especially important, it then explores the potential of collaborative virtual spaces in the therapy domain by asking "How can the potential of social virtual spaces be utilized to support and improve therapy encounters?" With regards to the first research question, the thesis presents two theoretical frameworks detailing different aspects of supporting smooth and varied collaboration processes. In addition, several user studies on the topic of collaborative virtual interaction are described, focusing on the role that different users can play during shared interaction and the effects that this distribution of roles and responsibilities has on both the performance and experience of the involved user pairs. The results presented for this first research question show that social virtual spaces have the potential to provide dedicated support for collaborative workflows. To enable users to adapt their working mode individually and as a team, interaction techniques should complement a team's natural interaction and communication. When presenting novel interactions to users, providing them with a way to support each other can ease their adaptation to these interactions. In these cases, the inclusion of all interested collaborators as active participators should be prioritized in order to let all users benefit from being immersed in a virtual environment. Addressing the combination of social virtual spaces with therapy in relation to the second research question, this thesis presents the result of a series of interviews with practicing physio- and psychotherapists. Motivated by the recorded expert feedback, it also reports on two more detailed explorations of specific areas of interest. The work presented for the second research question demonstrated the promise of using virtual environments in both exercise- and conversation-based therapy practice. Investigating the potential of shared interactions, the exploration of virtual recordings and the adaptation of virtual appearances, the presented work uncovered several topic areas that could be further explored regarding their possible use in the treatment of patients. Taken together, the six research articles presented in this thesis show both the value of supporting and understanding shared interactions in virtual spaces and their potential place in serious use-cases like the therapy domain. When introducing shared virtual environments to new user groups, the opportunity for mutual support through shared interaction techniques could be a crucial building block towards making virtual spaces both accessible and attractive to a variety of users.

The new millennium has been characterized by rising digitalization, the proliferation of shadow banking, and significant advancements in machine learning and natural language processing. These trends present both challenges and opportunities, which my dissertation addresses. This cumulative dissertation investigates critical aspects of financial stability, monetary policy, and the transition towards cashless economies through three distinct but interrelated studies. The first paper examines the risk-taking channel of monetary policy transmission within the euro area, focusing on shadow banks. Through vector autoregressive models, it assesses the impact of conventional and unconventional monetary policy shocks on shadow banks' asset growth and risk asset ratios. The results indicate that lower interest rates lead to a portfolio reallocation towards riskier assets and a general expansion of assets in shadow banks. In the case of conventional monetary policy shocks, both effects last three times as long as in the case of unconventional monetary policy shocks. Country-specific as well as sector-specific estimations confirm these findings. This study bridges gaps in the existing literature, especially in the eurozone, by highlighting the significant role shadow banks play in monetary policy transmission, suggesting implications for financial regulation and stability. The second paper explores the influence of financial stability considerations on US monetary policy, particularly during the Great Recession. Utilizing natural language processing and machine learning techniques on congressional hearings, this study constructs indicators for financial stability sentiment expressed by the Federal Reserve Chairs. Empirical analysis is conducted using Taylor-rule models, revealing that negative financial stability sentiment is associated with a more accommodative monetary policy stance, even before the Great Recession. This work provides new insights into the integration of financial stability concerns into monetary policy frameworks, demonstrating the need for a balanced approach to economic stability. The article suggests that under a dual mandate, such as that of the Federal Reserve, financial stability can, to some extent, already be factored into monetary policy deliberations. The third paper sheds new light on ``cash paradox'' by uncovering the factors of the cashless transition that has not been entirely understood so far. Using a comprehensive dataset across 65 countries, the study employs panel data models to explain the paradox (increasing demand for central bank money despite soaring digitalization), especially among technologically advanced countries, e.g., Japan. Empirical evidence suggests that digitalization is not significantly associated with higher reliance on physical cash. It uncovers a unique non-linear relationship between trust and cash usage (``Arch of Trust'') which holds after addressing potential endogeneity issues using 2SLS estimation. Opposed to the widespread misinterpretations of Keynes' (1937) reasons for holding cash, the findings highlight that distrust is the key factor unlocking two distinct puzzles in economics, linking cash hoarding with ``missing'' funds on capital markets and slower shift toward digital payments in low-trust societies. A key insight is the role of trust as a (social) insurance, cushion or safety net, dampening the perception of risk and reducing precautionary and transactionary demand for physical cash, while encouraging a shift towards riskier alternatives. This, in turn, is connected to the third puzzle, the ``paradox of prudence.'' A shift from riskier investments to safer assets, cash, may be prudent at the individual level but risky for the overall economy, a concern for macroprudential policymakers. Additionally, the research highlights the critical role of culture in driving the global movement towards cashless economies. Moreover, cultures that are more self-expression-oriented (which is the main cultural dimension) and culturally closer to Sweden are associated with less cash-intensive economies. These insights are vital for macroprudential regulators as well as for policymakers designing payment systems and CBDC in culturally diverse regions like the Eurozone. Collectively, these papers contribute to a deeper understanding of monetary policy, financial stability, and the transition from cash-based to (nearly) cashless societies, offering significant theoretical and practical implications for academics, regulators and central bankers.

The present study investigates the prosody of information-seeking (ISQs) and rhetorical questions (RQs) in Standard Chinese, in polar and wh-questions. Like in other languages, ISQs and RQs in Standard Chinese can have the same surface structure, allowing for a direct prosodic comparison between illocution types (ISQ vs RQ). Since Standard Chinese has lexical tone, the use of f0 as a cue to illocution type may be restricted. We investigate the prosodic differences between ISQs and RQs as well as the interplay of prosodic cues to RQs. In terms of f0, results showed that RQs were lower in f0, with the f0 range on the first word being expanded followed by f0 compression. RQs were further longer in duration and more often realized with non-modal voice quality (glottalized voice) as compared to ISQs. These prosodic cues were largely manipulated in tandem (illocutionary pairs with larger durational differences also showed larger differences in mean f0; voice quality, in turn, seemed to be an additional cue). We suggest three possible explanations (assertive force, focus, speaker attitude) that unite the present findings on RQs in Standard Chinese with the findings on RQs in other, non-tonal languages.

Entrepreneurship is recognized as an important discipline to achieve sustainable development and to address sustainability goals without losing sight of economic aspects. However, entrepreneurship rates are rather low in many industrialized countries with high income levels. Research clearly shows that there is a gap in the entrepreneurial process between intentions and subsequent actions. This means that not everyone with entrepreneurial ambitions also follows through and implements actions. This gap also exists for aspects of sustainability. As a result, there is a need to better understand the traditional and sustainability-focused entrepreneurial process in order to increase corresponding actions. This dissertation offers such a comprehensive perspective and sheds light on individual and contextual predictors for traditional and sustainability-focused behavior of entrepreneurs and self-employed across four studies. The first three studies focus on individual predictors. By providing a systematic literature review with 107 articles, Chapter 2 highlights the ambivalent role of religion for the entrepreneurial process. Relying on the theory of planned behavior (TPB) as theoretical basis, religion can have positive effects on entrepreneurial attitudes and behavioral control, but also negative consequences for other aspects of behavioral control and subjective norms due to religious restrictions. The quantitative empirical study in Chapter 3 similarly relies on the TPB and sheds light on individual perceptual factors influencing the sustainability-related intention-action gap in entrepreneurship. Using data from the 2021 Global Entrepreneurship Monitor (GEM) Adult Population Survey (APS) including 22,008 early-stage entrepreneurs from 44 countries worldwide, the results support our theoretical reasoning that sustainability-focused intentions are positively related to social entrepreneurial actions. In addition, it is demonstrated that positive perceptual moderators such as self-efficacy and knowing other entrepreneurs as role models strengthen this relationship while a negative perception such as fear of failure restricts social actions in early-stage entrepreneurship. The next quantitative empirical study in Chapter 4 examines the behavioral consequences of well-being at a sample of 6,955 German self-employed during COVID-19. This chapter builds on two complementary behavioral perspectives to predict how reductions in financial and non-financial well-being relate to investments in venture development. In this regard, reductions in financial well-being are positively related to time investments, supporting the performance feedback perspective in terms of higher search efforts under negative performance. In contrast, reductions in non-financial well-being are negatively related to time and monetary investments, yielding support for the broadening-and-build perspective indicating that negative psychological experiences narrow the thought-action repertoire and hinder resource deployment. The insights across these first three studies about individual predictors indicate that many different, subjective beliefs, perceptions and emotional states can influence the entrepreneurial process making entrepreneurship and self-employment highly individualized disciplines. The last quantitative empirical study provides an explorative view on a large number of contextual predictors for social and ecological considerations in entrepreneurial actions. Combining GEM data from 2021 on country level with further information from the World Bank and the OECD, a machine learning approach is employed on a sample of 84 countries worldwide. The results suggest that governmental and regulatory as well as cultural factors are relevant to predict social and ecological considerations. Moreover, market-related aspects are shown to be relevant predictors, especially socio-economic factors for social considerations and economic factors for ecological considerations. Overall, the four studies in this dissertation highlight the complexity of the entrepreneurial process being determined by many different individual and contextual factors. Due to the multitude of potential predictors, this dissertation can only give an initial overview of a selection of factors with many more aspects and interdependencies still to be examined by future research.