Soils in forest ecosystems bear a high potential as carbon (C) sinks in the mitigation of climate change. The amount and characteristics of soil organic matter (SOM) are driven by inputs, transformation, degradation and stabilization of organic substances. While tree species fuel the C cycle by producing aboveground and belowground litter, soil microorganisms are crucial for litter degradation as well as the formation and stabilization of SOM. Nonetheless, our knowledge about the tree species effect on the SOM status is limited, inconsistent and blurred. The investigation of tree species effects on SOM is challenging because in long-established forest ecosystems the spatial distribution of tree species is a result of the interplay of environmental factors including climate, geomorphology and soil chemistry. Moreover, tree distribution can further vary with forest successional stage and silvicultural management. Since these factors also directly affect the soil C-status, it is difficult to identify a pure “tree species effect” on the SOM status at regular forested sites. It therefore remains unclear in how far tree species-specific litter with different quality influences the microbial driven turnover and formation of SOM.
Tree species effects on SOM and related soil microbial properties were investigated by examining soil profiles (comprising organic forest floor horizons and mineral soil layers) in different forest stands at the recultivated spoil heap ‘Sophienhöhe’ located at the lignite open-cast mine Hambach near Jülich, Germany. The afforested sites comprised monocultural stands of Douglas fir (Pseudotsuga menziesii), black pine (Pinus nigra), European beech (Fagus sylvatica) and red oak (Quercus rubra) as well as a mixed deciduous stand site planted mainly with hornbeam (Carpinus betulus), lime (Tilia cordata) and common oak (Quercus robur) that were grown for 35 years under identical soil and geomorphological conditions. Because the parent material used for site recultivation was free from organic matter or coal material, the SOM accumulation is entirely the result of in situ soil development due to the impact of tree species.
The first study revealed that tree species had a significant effect on soil organic carbon (SOC) stocks, stoichiometric patterns of C, nitrogen (N), sulfur (S), hydrogen (H) and oxygen (O) as well as the microbial biomass carbon (MBC) content in the forest floor and the top mineral soil layers (0-5 cm, 5-10 cm, 10-30 cm). In general, forest floor SOC stocks were significantly higher at coniferous forest stands compared to deciduous tree species, whereas in mineral soil layers the differences were smaller. Thus, the impact of tree species decreased with increasing soil depth. By investigating the linkage of the natural abundance of 13C and 15N in the soil depth gradients with C:N and O:C stoichiometry, the second study showed that differences in SOC stocks and SOM quality resulted from a tree species-dependent turnover of SOM. Significantly higher turnover of organic matter in soils under deciduous tree species depended to 46 % on the quality of litterfall and root inputs (N content, C:N, O:C ratio), and on the initial isotopic signatures of litterfall. Hence, SOM composition and turnover also depends on additional – presumably microbially driven – factors. The subsequent results of the third study revealed that differences in SOM composition and related soil microbial properties were linked to different microbial communities. Phospholipid fatty acid (PLFA) patterns in the soil profiles indicated that the supply and availability of C and nutrient-rich substrates drive the distribution of fungi, Gram-positive (G+) bacteria and Gram-negative (G−) bacteria between tree species and along the soil depth gradients. The fourth study investigated the molecular composition of extractable soil microbial biomass-derived (SMB) and SOM-derived compounds by electrospray ionization Fourier transformation ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS). This was complemented by the analysis of nine monosaccharides representing microbial or plant origin. Microbially derived compounds substantially contributed to SOM and the contribution increased with soil depth. The supply of tree species-specific substrates resulted in different chemical composition of SMB with largest differences between deciduous and coniferous stands. At the same time, microorganisms contributed to SOM resulting in a strong similarity in the composition of SOM and SMB.
Overall, the complex interplay of tree species-specific litter inputs and the ability, activity and efficiency of the associated soil fauna and microbial community in metabolizing the organic substrates leads to significant differences in the amount, distribution, quality and consequently, the stability of SOM. These findings are useful for a targeted cultivation of tree species to optimize soil C sequestration and other forest ecosystems services.
Soil organic matter (SOM) is an indispensable component of terrestrial ecosystems. Soil organic carbon (SOC) dynamics are influenced by a number of well-known abiotic factors such as clay content, soil pH, or pedogenic oxides. These parameters interact with each other and vary in their influence on SOC depending on local conditions. To investigate the latter, the dependence of SOC accumulation on parameters and parameter combinations was statistically assessed that vary on a local scale depending on parent material, soil texture class, and land use. To this end, topsoils were sampled from arable and grassland sites in south-western Germany in four regions with different soil parent material. Principal component analysis (PCA) revealed a distinct clustering of data according to parent material and soil texture that varied largely between the local sampling regions, while land use explained PCA results only to a small extent. The PCA clusters were differentiated into total clusters that contain the entire dataset or major proportions of it and local clusters representing only a smaller part of the dataset. All clusters were analysed for the relationships between SOC concentrations (SOC %) and mineral-phase parameters in order to assess specific parameter combinations explaining SOC and its labile fractions hot water-extractable C (HWEC) and microbial biomass C (MBC). Analyses were focused on soil parameters that are known as possible predictors for the occurrence and stabilization of SOC (e.g. fine silt plus clay and pedogenic oxides). Regarding the total clusters, we found significant relationships, by bivariate models, between SOC, its labile fractions HWEC and MBC, and the applied predictors. However, partly low explained variances indicated the limited suitability of bivariate models. Hence, mixed-effect models were used to identify specific parameter combinations that significantly explain SOC and its labile fractions of the different clusters. Comparing measured and mixed-effect-model-predicted SOC values revealed acceptable to very good regression coefficients (R2=0.41–0.91) and low to acceptable root mean square error (RMSE = 0.20 %–0.42 %). Thereby, the predictors and predictor combinations clearly differed between models obtained for the whole dataset and the different cluster groups. At a local scale, site-specific combinations of parameters explained the variability of organic carbon notably better, while the application of total models to local clusters resulted in less explained variance and a higher RMSE. Independently of that, the explained variance by marginal fixed effects decreased in the order SOC > HWEC > MBC, showing that labile fractions depend less on soil properties but presumably more on processes such as organic carbon input and turnover in soil.