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Environmental DNA (eDNA) metabarcoding promises to be a cost- and time-efficient monitoring tool to detect interactions of arthropods with plants. However, observation-based verification of the eDNA-derived data is still required to confirm the reliability of those detections, i.e., to verify whether the arthropods have previously interacted with the plant. Here, we conducted a comparative analysis of the performance of eDNA metabarcoding and video camera observations to detect arthropod communities associated with sunflowers (Helianthus annuus, L.). We compared the taxonomic composition, interaction type, and diversity by testing for an effect of arthropod interaction time and occupancy on successful taxon recovery by eDNA. We also tested if prewashing of the flowers successfully removed eDNA deposition from before the video camera recording, thus enabling a reset of the community for standardized monitoring. We find that eDNA and video camera observations recovered distinct communities, with about a quarter of the arthropod families overlapping. However, the overlapping taxa comprised ~90% of the interactions observed by the video camera. Interestingly, eDNA metabarcoding recovered more unique families than the video cameras, but approximately two-thirds of those unique observations were of rare species. The eDNA-derived families were biased toward plant sap-suckers, showing that such species may deposit more eDNA than, for example, transient pollinators. We also find that prewashing of the flower heads did not suffice to remove all eDNA traces, suggesting that eDNA on plants may be more temporally stable than previously thought. Our work highlights the great potential of eDNA as a tool to detect plant-arthropod interactions, particularly for specialized and frequently interacting taxa.
This meta-scientific dissertation comprises three research articles that investigated the reproducibility of psychological research. Specifically, they focused on the reproducibility of eye-tracking research on the one hand, and studying preregistration (i.e., the practice of publishing a study protocol before data collection or analysis) as one method to increase reproducibility on the other hand.
In Article I, it was demonstrated that eye-tracking data quality is influenced by both the utilized eye-tracker and the specific task it is measuring. That is, distinct strengths and weaknesses were identified in three devices (Tobii Pro X3-120, GP3 HD, EyeLink 1000+) in an extensive test battery. Consequently, both the device and specific task should be considered when designing new studies. Meanwhile, Article II focused on the current perception of preregistration in the psychological research community and future directions for improving this practice. The survey showed that many researchers intended to preregister their research in the future and had overall positive attitudes toward preregistration. However, various obstacles were identified currently hindering preregistration, which should be addressed to increase its adoption. These findings were supplemented by Article III, which took a closer look at one preregistration-specific tool: the PRP-QUANT Template. In a simulation trial and a survey, the template demonstrated high usability and emerged as a valuable resource to support researchers in using preregistration. Future revisions of the template could help to further facilitate this open science practice.
In this dissertation, the findings of the three articles are summarized and discussed regarding their implications and potential future steps that could be implemented to improve the reproducibility of psychological research.
Aims: Fear of physical activity (PA) is discussed as a barrier to regular exercise in patients with heart failure (HF), but HF-specific theoretical concepts are lacking. This study examined associations of fear of PA, heart-focused anxiety and trait anxiety with clinical characteristics and self-reported PA in outpatients with chronic HF. It was also investigated whether personality-related coping styles for dealing with health threats impact fear of PA via symptom perception.
Methods and results: This cross-sectional study enrolled 185 HF outpatients from five hospitals (mean age 62 ± 11 years, mean ejection fraction 36.0 ± 12%, 24% women). Avoidance of PA, sports/exercise participation (yes/no) and the psychological characteristics were assessed by self-reports. Fear of PA was assessed by the Fear of Activity in Situations–Heart Failure (FActS-HF15) questionnaire. In multivariable regression analyses higher NYHA class (b = 0.26, p = 0.036) and a higher number of HF drugs including antidepressants (b = 0.25, p = 0.017) were independently associated with higher fear of PA, but not with heart-focused fear and trait anxiety. Of the three anxiety scores only increased fear of PA was independently associated with more avoidance behavior regarding PA (b = 0.45, SE = 0.06, p < 0.001) and with increased odds of no sports/exercise participation (OR = 1.34, 95% CI 1.03–1.74, p = 0.028). Attention towards cardiac symptoms and symptom distress were positively associated with fear of PA (p < 0.001), which explained higher fear of PA in patients with a vigilant (directing attention towards health threats) coping style (p = 0.004).
Conclusions: Fear of PA assessed by the FActS-HF15 is a specific type of anxiety in patients with HF. Attention towards and being distressed by HF symptoms appear to play a central role in fear of PA, particularly in vigilant patients who are used to direct their attention towards health threats. These findings provide approaches for tailored interventions to reduce fear of PA and to increase PA in patients with HF.
Au Luxembourg, des médiateur·trice·s scolaires externes apportent leur aide quand des conflits surgissent en milieu scolaire. Le Service de médiation scolaire fournit un soutien en cas de risque de décrochage scolaire et de conflits en lien avec l’inclusion et l’intégration d’élèves à besoins éducatifs spécifiques ou issu·e·s de l’immigration. Michèle Schilt s’est entretenue avec la directrice du service, Lis De Pina, sur le travail des médiateur·trice·s scolaires.