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The role of evolutionary and ecological processes with regard to the bauplan of anuran larvae remains little understood. We studied tadpoles of 144 lentic or lotic taxa in 22 families from seven zoogeographic regions. Using stacked high-resolution images, standardized examination of 30 morphological characters of preserved tadpoles (of body, tail, oral disc) were scored and compared to the impact of phylogenetic signal (multiple nuclear and mitochondrial markers) and macro-climate. Additionally, larvae were characterized as living in a lentic versus lotic environment and if nutrition specialist or not. Categorical Principal Component Analysis, distance matrices and pairwise correlation analyses were performed. Our results suggest that phylogeny has a higher impact on the morphology than habitat (i.e. lentic, lotic), while no effect of macro-climate or food specialization was found. We emphasize the value of highly standardized morphological data when investigating the influence of different factors of phenotypic variation.
This thesis seeks to improve the understanding of evolution and habitat as key factors forming tadpole morphology (i.e. of larvae of the order Anura), uncovers existing gaps in current research and recommends strategies and directions for future research. The present study improves the knowledge about the influences of evolution and habitat on the bauplan of tadpoles in a global scale ensuring maximum standardization and comparability of the data. In relation to the total number of tadpoles assumed to exist, only a small proportion has been described and only a few of them have been identified genetically. The lack of a global standard for their description makes it difficult to compare data. Using the tadpole of a harlequin frog (Atelopus) from Guiana region, it is shown that only an integrative approach with morphological and genetic data can solve taxonomic problems. In the study area of Madagascar, it becomes evident that in this region the common genetic history only has little influence on morphology, in contrast to the aquatic way of life. Tadpoles from flowing waters develop larger eyes, more robust tail muscles and smaller fins to cope better with current conditions and move more efficiently. In an additional study, the examination is extended to an almost global level. To achieve the intended standardization, over 1000 individuals (tadpoles) from 144 species have been examined. It can be shown that the common evolutionary history on a global scale influences morphology as strongly as the habitat. In addition, the influence of specialized nutrition and the climate is investigated.