Perennial crops eliminate soil disturbance and reduce the amount of synthetic chemicals that are applied to the soil, improving soil biodiversity and food web structure. Additionally, perennial cropping is characterised by all year-round surface coverage which benefits soil biota in terms of habitat and food sources. Perennial intermediate wheatgrass (Thinopyrum intermedium, IWG) was domesticated and commercialised by The Land Institute in Kansas as Kernza® and serves as an example for these nature-based solutions. It develops an extensive root system that has a higher nutrient retention, possibly reducing nutrient runoff. It thereby follows a more resource-conservative strategy with improved belowground-oriented resource allocation in its root system. This may reduce the need for excessive fertiliser as the crop has a higher nitrogen efficiency, among other things. IWG promoted the earthworm community and its diversity, more specifically, the occurrence of epigeic species (litter inhabitants), since those species benefit from the increased soil coverage and elimination of disturbances in the soil. As IWG creates a dense and extensive root system, as shown by the increased occurrence of root-feeding nematodes, endogeic species (horizontal burrowers) are supported through the provision of a reliable food source. IWG was characterised as a mostly undisturbed system with a highly structured food web through nematode analysis, as expressed through the promotion of structure indicators, for example, that are sensitive to disturbances in the soil and are therefore supported under no-till management. The root microbiome is continuously being shaped by the host as the crop regrows from the roots each vegetation period. This creates a symbiotic relationship and a beneficial feedback loop for the crop. Resultantly, the root-endophytic microbiome under IWG had a higher network complexity, connectivity and stability compared to annual wheat. The regrowth from the roots for IWG requires increased nutrient and energy storage, which was indicated by increased starch values. Correspondingly, the longer residence time of the roots in the soil resulted in higher lignin values. Furthermore, the decomposition pathway was dominated by fungivorous nematodes which may correspond to stimulated nutrient cycling and a heterogeneous resource environment, as seen for low input systems. Overall, perennial wheat cultivation improved soil biodiversity already after an establishment of 3-6 years. As those benefits were present for all three countries, the varying soil and climate conditions do not seem to interfere with the positive effect of perennial wheat on the soil ecosystem, demonstrating a wide transferability and adaptability of the crop onto other study sites as well. Enhanced complexity and connectivity of the food web in comparison to annual wheat may indicate a resistance against abiotic stress, suggesting IWG cultivation as a viable option for a sustainable and resilient agriculture. The improvement in nutrient cycling and the resource-efficient cultivation strategy for IWG could enable cultivation on marginal land where annual crop cultivation is not possible as the soils are susceptible to erosion and nutrient runoff. This opens up new possibilities for agricultural cultivation on previously unused land, thus contributing to food security in the future.

Introduction: Conventional agricultural land-use may negatively impact biodiversity and the environment due to the increased disturbances to the soil ecosystem by tillage, for example. Cultivation of the perennial grain intermediate wheatgrass (Thinopyrum intermedium, IWG, Kernza®) is a nature-based solution for sustainable agriculture, improving nutrient retention mainly through its extensive root system. Nematodes serve as sensitive bioindicators, detecting early changes in the soil food web, reflecting in changes in their community structure. Materials and Methods:IWG and annual wheat sites in South France, Belgium and South Sweden were investigated in April 2022 for two depths (5–15 cm; 25–35 cm) to evaluate the difference in nematode community structure among the cropping systems. Results: Sites with IWG cultivation held an accumulation of structure indicators (c-p 3–5 nematodes) compared to sites with annual wheat cultivation. A generalised linear mixed model revealed significantly more root feeders, especially for the subsoil, under IWG as a result of the perennial cultivation. The maturity index, plant-parasitic index, channel index and structure index were greater for IWG sites. The enrichment index was greater for annual wheat sites due to the dominance of bacterivores and enrichment indicators (c-p 1 nematodes). The nematode community structure (weighted faunal profile analysis) indicates IWG sites as being a generally undisturbed system with efficient nutrient cycling and balanced distribution of feeding types, as well as higher metabolic footprint values for root feeders (including plant-parasitic nematodes) and fungivores. Annual wheat sites, on the other hand, held indicators of a disturbed system with increased occurrence of opportunistic species and a more bacterial driven pathway. The topsoil had an increased occurrence of structure indicators in both cropping systems. Conclusion: IWG creates favourable conditions for a diverse food web, including improved nutrient cycling and a heterogeneous resource environment, regardless of climatic conditions, establishing it as a stable and resilient agricultural management system.