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Institut
- Raum- und Umweltwissenschaften (99) (entfernen)
Today obesity has been recognized as a disease. Evidence suggests that obesity often has Genetic, environmental, psychological and other factors. Growing evidence points to heredity as a strong determining factor of obesity. The characterization of uncoupling proteins (UCP) represents a major breakthrough of genetic factors towards understanding the molecular basis for energy expenditure and therefore likely to have important implication for the cause and treatment of human obesity. UCPs as mitochondrial anion carriers which creates a pathway that allows dissipation of the proton electrochemical gradient therefore which when deregulated are key risk factors in the development of obesity and other eating disorders. In order to better understand the roles of both UCP2 and UCP3 which considered as prime candidate genes involved in the pathogenesis of obesity, this study elucidate (1) Genomic organization: The human UCP2 (3) gene spans over 8.7 kb (7.5 kb) distributed on 8 (7) exons. Three UCP genes may have evolved from a common ancestor or are the result from gene duplication events. Two mRNA transcripts are generated from hUCP3 gene, the long and short form of hUCP3 is differing by the presence or absence of 37 amino acid residues at the C-terminus. (2) Mutational analysis revealed a mutation in exon 4 of hUCP2 resulting in the substitution of an alanine by a valine at codon 55 and an insertion polymorphism in exon 8 consisted of a 45 bp repeat located 150 bp downstream of the stop codon in the 3'-UTR. The allele frequencies of both polymorphisms were not significantly elevated in a subgroup of children characterized by low Resting Metabolic Rates (RMR). (3) Promoter Analysis showed that the promoter region of hUCP2 lacks a classical TATA or CAAT box. Functional characterization of hUCP2 promoter showed that minimal promoter activity was observed within 65 bp upstream of the transcriptional start site. 75 bp further upstream a strong cis-acting regulatory element was identified which significantly enhanced basal promoter activity. The regulation of human UCP2 gene expression involves complex interactions among positive and negative regulatory elements. the 5"-flanking region of the hUCP3 gene were characterized in which contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific MyoD and MEF2 sites. Functional characterization identified a cis-acting negative regulatory element between - 2983 and -982 while the region between -982 and -284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.
Hydrodynamic processes play a fundamental role in the distribution of salt within mangrove-fringed estuaries and mangrove forests. In this thesis, two hydrodynamic processes and their ecological implications were examined. (1) Passive Irrigation and Functional Morphology of Crustacean Burrows in Rhizophora-forests. The mangrove Rhizophora excludes more than 90% of the seawater salt at water intake at the roots. By means of conductivity methods and resin casting, it was found that crustacean burrows play a key role in the removal of excess salt from the root zone. Salt diffuses from the roots into the burrows, and is efficiently flushed from the burrows by rainwater infiltration and tidal irrigation. The burrows contribute significantly to favourable conditions for the growth of Rhizophora trees. (2) Trapping of Mangrove Propagules due to Density-driven Secondary Circulation in Tropical Estuaries. In North East Australian estuaries, mangrove propagules are drifted upstream by density-driven axial surface convergences. Propagules accumulate in hydrodynamic traps upstream from suitable habitat, where they are trapped at least for the entire tropical dry season. Axial convergences may provide an efficient barrier for propagule exchange across estuaries. In such estuaries, mangrove populations can be regarded as floristically isolated, not unlike island communities, even though the populations lie on a continuous coastline. This effect may contribute to the disjunct distribution observed in some mangrove species. The outcomes of this work contribute to the understanding of the importance of salt as a growth and habitat-restricting factor in the mangrove environment.
Until today the effects of many chlorinated hydrocarbons (e.g. DDT, PCBs) against the specific organisms are still a subject of controversial discussions. It was also the case for potential endocrine effects to influence the spermatogenesis correlated with possible changes of the population's vitality. To clear this situation, three questions could be at the centre of attention: 1) Do the chemicals cause a special harmful effect on the male reproductive tract? 2) Could some particular chemical mixtures act to bind and activate the human estrogen receptor (hER)? 3) Are the life stages of an organism specially sensitive to the effects of chemicals and therefore be established as Screening-Test-System? the connected effects of DDT and Arochlor 1254 as single substance and in 1:1 mixture according to their estrogenic effectiveness on zebrafish (Brachydanio rerio) were therefore investigated. the concentrations of the pesticides and their mixture ranged between 0.05-µg/l and 500-µg/l and separated by a factor of 10. It was turned out that the test concentrations of 500-µg/l were too toxic to zebrafish in all the cases. The experiment was followed up with four concentrations of DDT, A54 as well as their 1:1 mixture anew each separated by a factor of 10 and ranging between 0.05-µg/l and 50-µg/l. The bioaccumulation test within 8 days showed that the zebrafish accumulated the chemicals, but no equilibrum was reached and the concentration 0.05-µg/l was established as No Observed Effect Concentration (NOEC). Putting up on these analyses, the investigation of the life cycle (LC) starting with fertilized eggs demonstrated a reduction in the rate of hatchability, reproduction and length of fish emerged. These reductions involved the duration of the life cycle stages (LCS) which consequently lasted longer than expected. Exposure time and level of the tested chemicals accelerated the occurrence of these effects which were more significant when the chemical mixtures were used too. To establish whether the parameter assessed were correlated to the male reproductive tract, the quality, quantity and life span of sperm were assessed using the methods of Leong (1988) and Shapiro et al (1994). The sperm degeneration observed, led us to investigate the spermatogenesis and the ultrastructure of the testes. This last experiment showed a significant reduction of the late stage of spermatogenesis and the heterophagic vacuoles which play an important role in the spermatid maturation. It could therefore be concluded that, DDT and A54 could act synergically and cause disorders of the male reproductive tract of male zebrafish and influence also their growth.
Since November 1997, we started to focus on the population ecology of two sympatric Sinonatrix snakes in the Chutzuhu swamp, northern Taiwan. At the same time we also examined some specimens from Senckenberg Natural History Museum, Frankfurt am Main and accumulated field data of some observation made on S. percarinata suriki from Fushan botanical garden, Sanping and Gaoshu, Taiwan. According to the specimens examined, we suspect that the close phylogeny of S. percarinata suriki may come from two ancestors, northeast Taiwan population closest to Fujien or Zehjiang and the southwest population closest to Guandong or Vietnam. This pattern was also represented in some molecular phylogeny studies of freshwater fish in Taiwan. There were 22,462 trap-nights, taken from the Chutzuhu swamp, during the period November 1999 to September 2001 and 361 snakes were collected, comprising five species and 617 snake-times. The population sizes were based on the Lincoln-Peterson index and were estimated to be 988-±326 in S. annularis and 129-±78 in S. percarinata suriki. Movement and home range data showed S. annularis is a restricted activity water snake and S. percarinata suriki possesses great mobility in spatial patterns, but movement ability seems to be influenced by the size of the aquatic environment. S. annularis is live-bearing, on average 8.19 neonates and this principally occurs in September; S. percarinata suriki lays 6-24 eggs, but due to insufficient observations no conclusions can be drawn. It must be noted that oviposition was also noted in September. The reproductive mode may reflect on thermal requirement differences of the two sympatric snakes. S. annularis tended to be a fish (98%) eater and S. percarinata suriki take 50% fish and 50% frogs in their diet. Middle to high ground cover marshland appears to be the favorite microhabitat of S. annularis, and S. percarinata suriki seems prefer open creeks and ditches. The population condition of S. annularis in the Chutzuhu swamp seems to be rapidly deteriorating and this trend is also reflected in the BCI declines, low proportion stomach contents and diseases of S. annularis. Water seems to be the major influencing factor and strongly correlates with the conservation strategy. Conservation proposals for S. annularis in the Chutzuhu swamp will be formulated. During this study period we also developed an efficient technique for snake morphological data accumulation and image database, with the aid of the following devices, PC notebook and scanner, which is adapted for practical field studies. We also want to propose a component system for the establishment of a fundamental snake population databases (FPDS) for long-term snake ecological studies and monitoring herein.
This dissertation develops a rationale of how to use fossil data in solving biogeographical and ecological problems. It is argued that large amounts of fossil data of high quality can be used to document the evolutionary processes (the origin, development, formation and dynamics) of Arealsystems, which can be divided into six stages in North America: the Refugium Stage (before 15,000 years ago: > 15 ka), the Dispersal Stage (from 8,000 to 15,000 years ago: 8.0 - 15 ka), the Developing Stage (from 3,000 to 8,000 years ago: 3.0 - 8.0 ka), the Transitional Stage (from 1,000 to 3,000 years ago: 1 - 3 ka), the Primitive Stage (from 5,00 to 1,000 years ago: 0.5 - 1 ka) and the Human Disturbing Stage (during the last 500 years: < 0.5 ka). The division into these six stages is based on geostatistical analysis of the FAUNMAP database that contains 43,851 fossil records collected from 1860 to 1994 in North America. Fossil data are one of the best materials to test the glacial refugia theory. Glacial refugia represent areas where flora and fauna were preserved during the glacial period, characterized by richness in species and endemic species at present. This means that these (endemic) species should have distributed purely or primarily in these areas during the glacial period. The refugia can therefore be identified by fossil records of that period. If it is not the case, the richness in (endemic) species may not be the result of the glacial refugia. By exploring where mammals lived during the Refugium Stage (> 15 ka), seven refugia in North America can be identified: the California Refugium, the Mexico Refugium, the Florida Refugium, the Appalachia Refugium, the Great Basin Refugium, the Rocky Mountain Refugium and the Great Lake Refugium. The first five refugia coincide well with De Lattin- dispersal centers recognized by biogeographical methods using data on modern distributions. The individuals of a species are not evenly distributed over its Arealsystem. Brown- Hot Spots Model shows that in most cases there is an enormous variation in abundance within an areal of a species: In a census, zero or only a very few individuals occur at most sample locations, but tens or hundreds are found at a few sample sites. Locations where only a few individuals can be sampled in a survey are called "cool spots", and sites where tens or hundreds of individuals can be observed in a survey are called "hot spots". Many areas within the areal are uninhabited, which are called "holes". This model has direct implications for analyzing fossil data: Hot spots have a much higher local population density than cool spots. The chances to discover fossil individuals of a species are much higher in sediments located in a "hot spot" area than in a "cool spot" area. Therefore much higher MNIs (Minimum Number of Individuals) of the species should be found in fossil localities located in the hot spot than in the cool spot area. There are only a few hot spots but many cool spots within an areal of a single hypothetical species, consequently only a few fossil sites can provide with much high MNIs, whereas most other sites can only provide with very low MNIs. This prediction has been proved to be true by analysis of 70 species in FAUMAP containing over 100 fossil records. The temporal and spatial variation in abundance can be reconstructed from the temporospatial distribution of the MNIs of a species over its Arealsystem. Areas with no fossil records from the last thousands of years may be holes, and sites with much higher MNIs may be hot spots, while locations with low MNIs may be cool spots. Although the hot spots of many species can remain unchanged in an area over thousands of years, our study shows that a large shift of hot spots occurred mainly around 1,500-1,000 years ago. There are three directions of movement: from the west side to the east side of the Rockies, from the East of the USA to the east side of the Rockies and from the west side of the Rockies to the Southwest of the USA. The first two directions of shift are called Lewis and Clark- pattern, which can be verified with the observations mad by Lewis and Clark during their expedition in 1805-1806. The historical process of this pattern may well explain the 200-year-old puzzle why big game then abundant on the east side were rare on the west side of the Rocky Mountains noted by modern ecologists and biogeographers. The third direction of shift is called Bayham- pattern. This pattern can be tested by the model of Late Holocene resource intensification first described by Frank E. Bayham. The historical process creating the Bayham pattern will challenge the classic explanation of the Late Holocene resource intensification. An environmental change model has been proposed to account for the shift of hot spots. Implications of glacial refugia and hot spots areas for wildlife management and effective conservation are discussed. Suggestions for paleontologists and zooarchaeologists regarding how to provide more valuable information in their future excavation and research for other disciplines are given.
The collapse of the tailings pond of the Aznalcállar open pit mine (West of Seville, Spain) in April 1998 left more than 4000 ha of arable land and floodplains contaminated with heavy metal containing pyrite sludge. After a first remediation campaign a considerable contamination remained in the soil. The present study evaluates the possibilities of reflectance spectroscopy and airborne hyperspectral remote sensing for the qualitative and quantitative assessment of heavy metal contamination and the acidification risk related to the mining accident. Based on an extensive data set consisting of geochemical analyses and reflectance measurements of more than 300 soil samples different chemometrics methods (multiple linear regression, partial least squares and artificial neural networks) are tested for computation of concentrations of soil constituents on the basis of the spectral reflectance. Spectral mixture analysis is applied for the analysis of the spatial distribution of the contamination. The abundance information derived from spectral mixture analysis is turned into quantitative information incorporating an artificial mixture experiment. The results of this experiment provide a link between sludge abundance and sludge weight, allowing as a consequence calculation of the amount of residual sludge per pixel, the acidification potential and other parameters important for remediation planning. The application of laboratory, field and imaging spectroscopy for providing quantitative information about the contamination levels in their spatial context is a good complement to conventional methods. The advantage is the reduction of the time and labour-intensive geochemical analysis, because after the model calibration, further samples can be analysed directly with the chemometric models. Furthermore, the spatial distribution can be mapped with imaging spectroscopy data helping in a more precise remediation planning.
Two areas were selected to represent major process regimes of Mediterranean rangelands. In the County of Lagads (Greece), situated east of the city of Thessaloniki, livestock grazing with sheep and goats is a major factor of the rural economy. In suitable areas, it is complemented by agricultural use. The region of Ayora (Spain) is located west of the city of Valencia. It is one of regions most affected by fires in Spain. First of all, long time series of satellite data were compiled for both regions on the basis of Landsat sensors, which cover the time until 1976 (Ayora) and 1984 (Lagadas) with one image per year. Using a rigorous processing scheme, the data were geometrically and radiometrically corrected Specific attention was given to an exact sensor calibration, the radiometric intercalibration of Landsat-TM and "MSS. Proportional cover of photosynthetically active vegetation was identified as a suitable quantitative indicator for assessing the state of rangelands. Using Spectral Mixture Analysis (SMA) it was inferred for all data sets. The extensive data base procured this way enabled to map fire events in the Ayora area based on sequential diachronic sets and provide fire dates, perimeter as well as fire recurrence for each pixel. The increasing fire frequency in the past decades is in large parts attributed to the accelerated abandonment of the area that leads to an encroachment of shrublands and the accumulation of combustible biomass. On the basis of the fire mapping results, a spatial and temporal stratification of the data set allowed to asses plant recovery dynamics on the landscape level through linear trend analysis. The long history of fire events in the Mediterranean frequently leads to processes of auto-succession. Following an initial dominance of herbaceous vegetation this commonly leads to similar plant communities as the ones present before the fire. On a temporal axis, this results in typical exponential post-fire trajectories which could also be shown in this study. The analysis of driving factors for post-fire dynamics confirmed the importance of aspect and slope. Locations with lower amounts of solar irradiation and favourable water supply yielded faster recovery rates and higher post-fire vegetation cover levels. In most cases, the vegetation cover levels observed before the fire were not reached within the post-fire observation period. In the area of Lagadas, linear trend analysis and additional statistical parameters were used to infer a degradation index. This could be used to illustrate a complex pattern of stability, regeneration and degradation of vegetation cover. These different processes and states are found in close proximity and are clearly determined by topography and elevation. Following a sequence of analyses, it was found that in particular steep, narrow valleys show positive trends, while negative trends are more abundant on plain or gently undulating areas. Considering the local grazing regime, this spatial differentiation was related to the accessibility of specific locations. Subsequently, animal numbers on community level were used to calculate efficient stocking rates and assess the temporal development of their relation with vegetation cover. This calculation of temporal trajectories illustrated that only some communities show the expected negative relation. To the contrary, a positive relation or even changing relation patterns are observed. This signifies recent concentration and intensification processes in the grazing scheme, as a result of which animals are kept in sheds, where additional feedstuffs are provided. In these cases, free roaming of livestock animals is often confined to some hours every day, which explains the spatial preference of easily accessible areas by the shepherds. Beyond these temporal trends, it was analysed whether the grazing pattern is equally reflected in a spatial trend. Making use of available geospatial information layers, the efforts required to reach each location was expressed as a cost. Then, cost zones could be defined and woody vegetation cover as a grazing indicator could be inferred for the different zones. Animal sheds were employed as starting features for this piospheric analysis, which could be mapped from very high spatial resolution Quickbird image data. The result was a clearly structured gradient showing increasing woody vegetation cover with increasing cost distance. On the basis of these two pilot studies, the elements of a monitoring and interpretation framework identified at the beginning of the work were evaluated and a formal interpretation scheme was presented.
The main goal of this publication is the development and application of an empirical method, which allows to forecast the transport of radionuclides in soils ad sediments. The calculations are based on data published in the literature. 10 case studies, comprising 30 time series, deal with the transport of Cs-134, Cs-137, Sr-85, Sr-90, and Ru-106. Transport in undisturbed soils and experimental systems like lysimeters and columns in laboratories are dealt with. The soils involved cover a large range of soils, e. g. podsols, cambisols (FAO), and peaty soils. Different speciations are covered, namely ions, aerosols, and fuel particles. Time series analysis centres around the Weibull-distribution. All theoretical models failed to forecast the transport of radionuclides. It can be shown that the parameters D and v, the dispersion coefficient and the advection velocity, appearing in solutions of the advection-dispersion equation (ADE), have no real physical meaning. They are just fitting parameters. The calculation of primary photon fluence rates, caused by Cs-137 in the soil, stresses the unreliability of forecasts based on theoretical models.
This study investigates the endemic centres of Indonesian animals and the biodiversity across geographical gradients. At the same time, it also evaluated different lines suggested for separating the Oriental and Australian faunal region in the Indonesian region. The analyses have mainly used the present-day distribution of terrestrial vertebrates, especially the smallest ranges of species and subspecies. The results show that faunal migration of Oriental and Australian lineages to the Indonesian Archipelago may have been happening since the Palaeocene period and more importantly, island drifts might have facilitated such migration. These events caused major reorganisation of island positions and island forms, which in turn resulted in faunal extinction around the mid-Pliocene. Some islands, especially in the Wallacea region, emerged very late and as a result nowadays they are lacking endemic forms. There are currently at least seven endemic centres, which can be recognised, i.e. Borneo, Java, Sumatra, Sulawesi, North Moluccas, New Guinea and the Lesser Sundas/Banda Arcs. The affinities between these endemic centres revealed that there are two clusters of islands in the Indonesian Archipelago. These different clusters suggest in turn the shifts of biogeographical lines in the Indonesian Archipelago. Furthermore, oscillation in climate, eustatic sea level changes and fluctuations in vegetation in the Quaternary period had much affected the distribution pattern of animals. There was a phase of expansion for montane oak forests, grasslands and woodlands during the period 18,000-14,000 years ago in East Indonesia and 16,500-12,000 years ago in West Indonesia. Such an expansion led to the increased isolation of rainforests and of the faunas adapted to them. These periods are also indicated by the lowering of the tree line which facilitated montane fauna to disperse across lower elevations. At 8,000-9,000 years ago, the climate became warmer and slightly wetter. The mid- to upper montane forests expanded to their full altitudinal range, while montane oak forest, grassland, and woodland areas had contracted. The oscillation in climate, eustatic sea level changes and fluctuations in vegetation in turn determines much the formation of numerous sub endemic centres, which today can be found within the mainland. Recently, there are 14 sub endemic centres on Borneo, 8 on Java, 16 on Sumatra, 14 on Sulawesi and 14 on New Guinea. From the conservation management point of view, the identification of such sub endemic centres would generate valuable information for the protection effort.
The fragmentation of landscapes has an important impact on the conservation of biodiversity. The genetic diversity is an important factor for a population- viability, influenced by the landscape structure. However, different species with differing ecological demands react rather differently on the same landscape pattern. To address this feature, we studied ten xerothermophilous butterfly species with differing habitat requirements (habitat specialists with low dispersal power in contrast to habitat generalists with low dispersal power and habitat generalists with higher dispersal power). We analysed allozyme loci for about 10 populations (Ã 40 individuals) of each species in a western German study region with adjoining areas in Luxemburg and north-eastern France. The genetic diversity and genetic differentiation between local populations was discussed under conservation genetic aspects. For generalists we detected a more or less panmictic structure and for species with lower abundance and sedentarily behaviour the effect of isolation by distance. On the other hand, the isolation of specialists was mostly reflected by strong genetic differentiation patterns between the investigated populations. Parameters of genetic diversity were mostly significantly higher in generalists, compared to specialists. Substructures within populations as an answer of low intrapatch migration, low population densities and high population fluctuations could be shown as well. Aspects of landscape history (the historical distribution of habitats resulting of the presence of limestone areas) and the changes of extensive sheep pasturing and the loss of potential habitats in the last few decades (recent fragmentation) are discussed against the gained genetic data-set of the ten butterflies.
It has been the overall aim of this research work to assess the potential of hyperspectral remote sensing data for the determination of forest attributes relevant to forest ecosystem simulation modeling and forest inventory purposes. A number of approaches for the determination of structural and chemical attributes from hyperspectral remote sensing have been applied to the collected data sets. Many of the methods to be found in the literature were up to now just applied to broadband multispectral data, applied to vegetation canopies other than forests, reported to work on the leaf level or with modelled data, not validated with ground truth data, or not systematically compared to other methods. Attributes that describe the properties of the forest canopy and that are potentially open to remote sensing were identified, appropriate methods for their retrieval were implemented and field, laboratory and image data (HyMap sensor) were acquired over a number of forest plots. The study on structural attributes compared statistical and physical approaches. In the statistical section, linear predictive models between vegetation indices derived from HyMap data and field measurements of structural forest stand attributes were systematically evaluated. The study demonstrates that for hyperspectral image data, linear regression models can be applied to quantify leaf area index and crown volume with good accuracy. For broadband multispectral data, the accuracy was generally lower. The physically-based approach used the invertible forest reflectance model (INFORM), a combination of well established sub-models FLIM, SAIL and LIBERTY. The model was inverted with HyMap data using a neural network approach. In comparison to the statistical approach, it could be shown that the reflectance model inversion works equally well. In opposition to empirically derived prediction functions that are generally limited to the local conditions at a certain point in time and to a specified sensor type, the calibrated reflectance model can be applied more easily to different optical remote sensing data acquired over central European forests. The study on chemical forest attributes evaluated the information content of HyMap data for the estimation of nitrogen, chlorophyll and water concentration. A number of needle samples of Norway spruce were analysed for their total chlorophyll, nitrogen and water concentrations. The chemical data was linked to needle spectra measured in the laboratory and canopy spectra measured by the HyMap sensor. Wavebands selected in statistical models were often located in spectral regions that are known to be important for chlorophyll detection (red edge, green peak). Predictive models were applied on the HyMap image to compute maps of chlorophyll concentration and nitrogen concentration. Results of map overlay operations revealed coherence between total chlorophyll and zones of stand development stage and between total chlorophyll and zones of soil type. Finally, it can be stated that the hyperspectral remote sensing data generally contains more information relevant to the estimation of the forest attributes compared to multispectral data. Structural forest attributes, except biomass, can be determined with good accuracy from a hyperspectral sensor type like HyMap. Among the chemical attributes, chlorophyll concentration can be determined with good accuracy and nitrogen concentration with moderate accuracy. For future research, additional dimensions have to be taken into account, for instance through exploitation of multi-view angle data. Additionally, existing forest canopy reflectance models should be further improved.
In past years, desertification and land degradation have been acknowledged as a major threat to human welfare world-wide, and their environmental and societal implications have sparked the formulation of the UN Convention to Combat Desertification (UNCCD). Any measure taken against desertification, or the design of dedicated early warning systems, must take into account both the spatial and temporal dimensions of process driving factors. Equally important, past and present reactions of ecosystems to physical and socio-economical disturbances or management interventions need to be understood. In this context, remote sensing and geoinformation processing support the required assessment, monitoring and modelling approaches, and hence provide an essential contribution to the scientific component of the struggle against desertification. Supported by DG Research of the European Commission, the Remote Sensing Department of the University of Trier convened RGLDD to promote scientific exchange between specialists working on the interface of remote sensing, geoinformation processing, desertification/land degradation research and its socio-economic implications. Although targeted at the scientific community, contributions with application perspectives were of crucial importance and both an overview of the current state of the art as well as operational opportunities were presented. Hosted at the Robert-Schuman Haus in Trier, the conference gained widespread attention and attracted an international audience from all parts of the world, which underlines the global dimension of land degradation and desertification processes. Based on a rigorous review of submitted abstracts, more than 100 contributions were accepted for oral and poster presentation, which are found in these proceedings edition in full paper form. Please note: This document is optimised for screen resolution, to receive a high-resolution version please contact the editors.
The allergic contact dermatitis (ACD) to small molecular weight compounds is a common inflammatory skin reaction. ACD is restricted to industrialized countries, has an enormous sociomedical and socioeconomic impact. About 2,800 compounds from the six million chemicals known in our environment are believed to have allergic, and to a lesser degree also contact-sensitizing or immunogenic properties causing allergic contact dermatitis. ACD results from T cell responses to harmless, low molecular weight chemicals (haptens) applied to the skin. Haptens are not directly recognized by the cells of the immune system. They need to be presented by subsets of antigen presenting cells to the cells of the immune system. In this regard, epidermal Langerhans cells (LC) and the cells into which they mature (dendritic cells) are believed to play a pivotal role in the sensitization process for ACD. LC are able to bind the haptens, internalize them, and present them to naive T cells and induce thereby the development of effector T cells. They are so-called professional antigen presenting cells. This process is initiated and maintained by the release of several mediators, which are released by various cells after their contact with the haptens. One of the first proteins secreted into the environment is interleukin (IL)-1ß. This cytokine is produced and secreted minutes after an antigen enters the cell. It is commonly believed that the large amounts of this protein and other cytokines such as granulocyte-colony stimulation factor (GM-CSF) and tumor necrosis factor alpha (TNF-ï¡) needed for the initiation and activation of ACD are coming first from other cells residing in the skin, e.g., keratinocytes, monocytes and macrophages. These cytokines provide the danger signals needed for the activation of the Langerhans cell (LC), which then produce via a positive feedback loop various cytokines themselves. In addition, other proteins such as chemokines influence the generation of danger signals, migration, homing of T cells in the local lymph nodes as well as the recruitment of T cells into the skin. Thus, a small molecular compounds or hapten needs to be able to induce danger signals in order to become immunogenic. In this study, we investigated whether para-phenylenediamine (PPD), an arylamine and common contact allergen, is able to induce danger signals and likely provide the signals needed for an initiation of an immune response[162, 163]. PPD is used as an antioxidant, an ingredient of hair dyes, intermediate of dyestuff, and PPD is found in chemicals used for photographic processing. But up to date, it has not been clearly demonstrated if PPD itself is a sensitizing agent. Thus, this study aimed on the potential of PPD to provide the danger signals by studying IL-1β, TNF-ï¡, and monocyte chemoattractant proteins (MCP-1) in human monocytes, peripheral blood mononuclear cells (PBMC) from healthy volunteers, and also in two human monocyte cell lines namely U937, and THP-1. This study found that PPD decreased dose- and time-dependently the expression and release of three relevant mediators involved in the generation of danger signals. Namely, PPD reduced the mRNA and protein levels for IL-1ß, TNF-ï¡, and MCP-1 in primary human monocytes from various donors. These findings were extended and validated by investigations using the cell line U937. The data were highly specific for PPD, and no such results were gained for its known auto oxidation product called Bandrowski- base or for meta-phenylenediamine (MPD), and ortho-phenylenediamine (OPD). Therefore, we can speculate that this effect is likely to be dependent on the para-substitution. Based on these results we conclude that PPD itself is not able to mount a cascade for the induction of danger signals. It should be mentioned that it is still possible that PPD induces danger signals for sensitization by other unknown processes. Therefore, more research is still needed focusing on this subject especially in professional antigen presenting cells in order to solve the still open question whether PPD itself sensitizes naive T cells or if PPD is solely an allergen. Independently we found unexpectedly that PPD as well as other haptens such as 2, 4-Dinitrochlorobenzene, nickelsulfate, as well as some terpenoide increased clearly the expression of CC chemokin receptor 2 (CCR2), the receptor for the chemokine MCP-1. Up to date, the main importance for the CCR2 receptor comes from results demonstrating that CCR2 is critical for the migration of monocytes after encounter with bacterial lipopolysaccharides. Under these circumstances the receptor disappears from the cell surface and is down regulated. An up regulation of CCR2 has not been reported for haptens, and deserves further investigations.
In addition to flood disasters on major rivers, damage caused by the flooding of smaller and medium-sized tributaries is also of considerable significance. To ensure that flood protection measures are effective, engineering flood prevention measures on the rivers must be supported by integrated catchment management. This includes decentralised water retention measures implemented in the sectors of forestry, agriculture and in residential areas. Within this scope new instruments have to be elaborated and introduced, such as GIS-based systems and systems for the evaluation of economic consequences and eco-efficiency of flood damage precaution measures associated with land-use. These are extremely significant for improving information management, the prevention of advice to the general public and for the acceptance of flood precaution measures. The conference intends to promote scientific exchange between specialists working on all areas concerning integrated catchment management. This includes the methodology for identification of catchment types prone to flooding hazards, the control and validation of land-use concepts for decentralised water retention as well as its combination and upscaling procedures up to mesoscale catchments. As catchment management is not only the concern of natural scientists the strategies for enhancing catchment management and the development of decision-support tools will also be important topics of the conference. ***Addenda *1. The articles from page 136 to 161 belong to session 5 *2. Article page 107: Ancient irrigation strategies: land use and hazard mitigation in Ma-´rib, Yemen (New list of authors: Ueli Brunner (a) , Michael Schütz (b), Dana Pietsch (c), Peter Kühn (c), Thomas Scholten (c), Iris Gerlach (d))
Tropospheric ozone (O3) is known to have various detrimental effects on plants, such as visible leaf injury, reduced growth and premature senescence. Flux models offer the determination of the harmful ozone dose entering the plant through the stomata. This dose can then be related to phytotoxic effects mentioned above to obtain dose-response relationships, which are a helpful tool for the formulation of abatement strategies of ozone precursors. rnOzone flux models are dependant on the correct estimation of stomatal conductance (gs). Based on measurements of gs, an ozone flux model for two white clover clones (Trifolium repens L. cv Regal; NC-S (ozone-sensitive) and NC-R (ozone-resistant)) differing in their sensitivity to ozone was developed with the help of artificial neural networks (ANNs). White clover is an important species of various European grassland communities. The clover plants were exposed to ambient air at three sites in the Trier region (West Germany) during five consecutive growing seasons (1997 to 2001). The response parameters visible leaf injury and biomass ratio of NC-S/NC-R clone were regularly assessed. gs-measurements of both clones functioned as output of the ANN-based gs model, while corresponding climate parameters (i.e. temperature, vapour pressure deficit (VPD) and photosynthetic active radiation (PAR)) and various ozone concentration indices were inputs. The development of the model was documented in detail and various model evaluation techniques (e.g. sensitivity analysis) were applied. The resulting gs model was used as a basis for ozone flux calculations, which were related to above mentioned response parameters. rnThe results showed that the ANNs were capable of revealing and learning the complex relationship between gs and key meteorological parameters and ozone concentration indices. The dose-response relationships between ozone fluxes and visible leaf injury were reasonably strong, while those between ozone fluxes and NC-S/NC-R biomass ratio were fairly weak. The results were discussed in detail with respect to the suitability of the chosen experimental methods and model type.
The skin is continuously challenged by environmental antigens that may penetrate and elicit a skin sensitization, which can develop into allergic contact dermatitis. Medical treatment for allergic contact dermatitis is limited - in fact only acute symptoms can be cured and for secondary prevention of the disease a lifelong avoidance of the allergen(s) is necessary. Therefore, the screening of the sensitization potential of substance used in commercially available products is indispensable to prevent such diseases. Hence, risk assessment is deduced from data obtained by murine local lymph node assay predominantly, but there exists a need to develop methods capable of providing the same information that do not require the use of animals in view of legislative initiatives such as REACH (registration, evaluation, authorization of chemicals) as well as the 7th Amendment to the Cosmetics Directive (2003/15/EC). Therefore, a number of promising in silico and in vitro approaches are being developed to address this need. In vitro test systems using the response of dendritic cells, which are the key player in the elicitation process of contact dermatitis, are established, but, although these novel methods for hazard identification might find application in the context of screening, it is not clear whether these approaches are useful for the purposes of risk assessment and risk management to predict allergic potency. Therefore, it was investigated whether on the one hand in vitro generated dendritic cells from primary blood monocytes (MoDC) and on the other hand a continuous monocytic cell line, the THP-1 cells, suggested as dendritic cell surrogate, react to a presumably weak allergen. Ascaridol, predicted as one of the possible causes for tea tree oil contact dermatitis, was studied and its effects in these two in vitro skin sensitization models were explored. Thus, the surface expression of CD86, HLADR, CD54, and CD40, which are known as activation markers in both in vitro models, were measured via flow cytometry. For MoDC, an augmented CD86 and HLADR surface expression in comparison to untreated cells were determined after 24 h exposure with ascaridol. An increased CD54 and CD40 surface expression were found only in some donors. After long term incubation of 96 h, ascaridol-treated MoDC still up-regulated CD86 and additionally an augmented CD40 expression was measured in all studied donors. An enhanced CD54 expression was determined for 50 percentage of all investigated donors. Furthermore, CD80, CD83 and CD209 protein expression were up-regulated in MoDC after 96 h of ascaridol incubation. In addition, it was determined that after 24 h ascaridol-treated MoDC showed an increased capacity to uptake antigens, whereas after 96 h this capacity got lost and antigen-capturing devices were reduced in comparison to non-treated MoDC. Moreover, the cytokine release of ascaridol-treated MoDC were measured after 24 h. Tumor necrosis factor (TNF)alpha, interleukin (IL)-1beta and IL 6 secretion were determined in some donors. Furthermore, IL-8 release was clearly increased after 24 h ascaridol treatment. By the same token, THP-1 cells were analyzed after ascaridol treatment for several activation markers. We found a similar response pattern as measured in MoDC. Ascaridol induced CD86 expression as well as CD54 after 24 h incubation. Additionally, the impact of ascaridol on phosphorylation of p38 mitogen-activated protein kinase, which had been shown to be involved in increased expression of activation markers like CD86 by others, were studied via Western blot analysis. A phosphorylation of p38 was determined after 15 min of ascaridol stimulation. Moreover, an augmented CD40 and HLADR surface expression were measured in a dose-response manner after 24 h ascaridol treatment. Also similar to MoDC an enhanced IL-8 secretion after ascaridol stimulation was observed in THP-1 cells. Hence, for the first time it was shown that ascaridol has immuno-modulating effects. The obtained data from both in vitro systems, MoDC and THP-1 cells, identified ascaridol as a sensitizer. Although for both systems there remain significant challenges to overcome for potency assessment, ascaridol is presumed to be a weak sensitizer probably. Interestingly, ascaridol treatment of THP-1 cells resulted also in an increased augmentation of CD184 and CCR2, two chemokine receptors expressed on monocyte. Therefore, these data encouraged the exploration of chemokine receptors as tools in skin sensitization prediction. Consequently, the combination of chemical assays with in vitro techniques may provide a useful surrogate to animal testing for skin sensitization. Due to the continuously changing environmental conditions, it is necessary to regularly monitor and update the spectrum of sensitizers that elicit contact dermatitis. Therefore, both debated in vitro test systems will become indispensable tools.
As an interface between an individual and its environment, the skin is a major site of direct exposure to exogenous substances. Once absorbed, these substances may interact with different biomolecules within the skin. The aryl hydrocarbon receptor (AhR) signaling pathway is one mechanism whereby the skin responds to exposures, predominantly through the induction or upregulation of metabolizing enzymes. One known physiological role of the AhR in many tissues is its involvement in the control of cell cycle progression. In skin, almost nothing is known about this physiological function. Moreover, the question whether frequently used naturally occurring phenolic derivatives like eugenol and isoeugenol impact on the AhR within the skin has rarely been studied so far. Eugenol and isoeugenol are due to their odour referred to as fragrances. The ubiquitous distribution of eugenol and isoeugenol results in an almost unavoidable contact with these substances in our daily lives. Despite this fact, their molecular mechanisms of action in skin are poorly understood. There is evidence supporting the hypothesis that these substances may impact on the AhR. On the one hand, eugenol is shown to induce cytochrome P450 1A1 (CYP1A1), a well-known target gene of the AhR. On the other hand, their known anti-proliferative properties might also be mediated by the AhR, based on its physiological function. In order to proof this hypothesis, it was investigated whether eugenol and isoeugenol impact on the AhR signaling pathway in skin cells. Results revealed that eugenol as well as isoeugenol impact on the AhR signaling pathway in skin cells. Both substances caused the translocation of the AhR into the nucleus, induced the expression of the well-known AhR target genes CYP1A1 and AhR repressor (AhRR) and exhibited impact on cell cycle progression. Both substances caused an AhR-dependent cell cycle arrest in skin cells, modulated protein levels of several cell cycle regulatory proteins, inhibited DNA synthesis and thereby reduced cell numbers. The comparison of wildtype cells to AhR knockdown cells revealed an influence of the AhR on cell cycle progression in skin cells in the absence of exogenous ligands. AhR knockdown cells exhibited a slower progression through the cell cycle caused by an accumulation of cells in the G0/G1 phase of the cell cycle and a decreased DNA synthesis rate. Modulation of cell cycle regulatory proteins involved in the transition from the G0/G1 to the S phase of the cell cycle was altered in AhR knockdown cells as well. To conclude, eugenol as well as isoeugenol were able to impact on the AhR signaling pathway in skin cells. Their molecular mechanisms of action are similar to those of classical AhR ligands, although their structural characteristics strongly differ from that of these ligands. In the absence of exogenous ligands the AhR promotes cell cycle progression in many tissues and this knowledge could be expanded on skin-derived cells within the scope of this thesis.
The reduction of information contained in model time series through the use of aggregating statistical performance measures is very high compared to the amount of information that one would like to draw from it for model identification and calibration purposes. It is readily known that this loss imposes important limitations on model identification and -diagnostics and thus constitutes an element of the overall model uncertainty as essentially different model realizations with almost identical performance measures (e.g. r-² or RMSE) can be generated. In three consecutive studies the present work proposes an alternative approach towards hydrological model evaluation based on the application of Self-Organizing Maps (SOM; Kohonen, 2001). The Self-Organizing Map is a type of artificial neural network and unsupervised learning algorithm that is used for clustering, visualization and abstraction of multidimensional data. It maps vectorial input data items with similar patterns onto contiguous locations of a discrete low-dimensional grid of neurons. The iterative training of the SOM causes the neurons to form a discrete, data-compressed representation of the high-dimensional input data. Using appropriate visualization techniques, information on distributions, patterns and relationships in complex data sets can be extracted. Irrespective of their potential, SOM applications have earned very little attention in hydrological modelling compared to other artificial neural network techniques. Therefore, the aim of the present work is to demonstrate that the application of Self-Organizing Maps has very high potential to address fundamental issues of model evaluation: It is shown that the clustering and classification of model time series by means of SOM can provide useful insights into model behaviour. In combination with the diagnostic properties of Signature Indices (Gupta et al., 2008; Yilmaz et al., 2008) SOM provides a novel tool for interpreting the model parameters in the hydrological context and identifying parameter sets that simultaneously meet multiple objectives, even if the corresponding model realizations belong to different models. Moreover, the presented studies and reviews also encourage further studies on the application of SOM in hydrological modelling.
This thesis presents a study of tsunami deposits created by the 2004 Indian Ocean tsunami at the Thai Andaman coast. The outcomes of a study are the characteristics of tsunami deposit for paleo-tsunami database, the identification of major sediment layers in tsunami deposit and the reconstructing tsunami run-ups from the characteristics of tsunami deposit for a coastal development program. The investigations of tsunami deposit are made almost 3 years after the event. Field investigations characterize the tsunami deposit as a distinct sediment layer variable in thickness of gray sand deposited with an erosional basis on a pre-existing soil. The best location for the observation of recent tsunami deposit is the area located about 50-200 m inland from the coastline. In most cases, the deposit layer is normally graded. In some cases, the deposit contains rip-up clasts of muddy soils and/or organic matters. The tsunami deposits are compared with three deposits from coastal sub-environments. The mean grain-size and standard deviation of deposits show that the shoreface deposits are fine to very fine sand, poorly to moderately well sorted; the swash zone deposits are coarse to fine sand, poorly to well sorted; the berm/dune deposits are medium to fine sand, poorly to well sorted; and the tsunami deposits are coarse to very fine sand, poorly to moderately well sorted. The plots of deposit mean grain-size versus sorting indicate that the tsunami deposits are composed of shoreface deposits, swash zone deposits and berm/dune deposits as well. The vertical variation of the texture of tsunami deposit shows that the mean grain-size fines upward and fining landward. The analysis and interpretation of the run-up numbers from the characteristics of tsunami deposits get three run-ups for the 2004 Indian Ocean tsunami at the Thai Andaman coast. It corresponds to field observations from the eye-witness reports and local people- affirmations. The total deposition is a major transportation pattern of onshore tsunami sediments. The sediments must fine in the direction of transport. In general, the major origins of the sediment are the swash zone and berm/dune zone where coarse to medium sand is a significant material, the minor origin of tsunami sediment is a shoreface where a significant material is fine to very fine sand. Only at an area with flat slope shorface, the major origin of tsunami sediment is the shoreface. The thicknesses, the mean grain-sizes, and the standard deviations of tsunami deposits are used to evaluate the influences of coastal morphology on the sediment characteristics. The evaluations show that the tsunami affected areas were attacked by the variable energy waves. Wave energies at the direct tsunami wave affected areas are higher than at the indirect tsunami wave affected areas. Tsunami wave energy is highly dissipated at an area with steep slope shoreface. In the same way, tsunami run-up energy is highly dissipated at an area with steep slope onshore. A channel paralleled to the coastline decreases the run-up velocity, slightly dissipates run-up energy. The road and pond highly influence the characteristics of tsunami deposit and tsunami run-up. A road obstructs the run-up velocity, dissipates run-up energy. A pond decreases run-up velocity, dissipates run-up energy. The characteristics of tsunami deposit can be interpreted for reconstructing the characteristics of tsunami run-up such as a run-up height and a flow velocity. Soulsby et al.(2007)- model is applied for reconstructing tsunami run-up at the study areas. The input parameters are sediment grain-size and sediment inundation distance. Ao Kheuy beach and Khuk Khak beach, Phang Nga province, Thailand are the areas listed for reconstructing tsunami run-up. The evaluated run-up heights are 4.2-4.9 m at Ao Kheuy beach, and 5.4-9.4 m at Khuk Khak beach. The evaluated run-up velocities are 12.8-19.2 m/s (maximum) and 0.2-1.9 m/s (mean) at the coastline and onshore, respectively. Hence, a reasonably good agreement between the evaluated and observed run-up is found. Tsunami run-up height and velocity can be used for coastal development and risk management in the tsunami affected areas. The case studies from the Thai Andaman coast suggest that in the area from coastline to about 70-140 m inland was flooded by the high velocity (high energy) run-ups, and those run-up energies were dissipated there. That area ought to be a non-residential area or a physical protection construction area (flood barrier, forest planting, etc.).
During and after application, pesticides enter the atmosphere by volatilisation and by wind erosion of particles on which the pesticide is sorbed. Measurements at application sites revealed that sometimes more than half of the amount applied is lost into the atmosphere within a few days. The atmosphere is an important part of the hydrologic cycle that can transport pesticides from their point of application and deposit them into aquatic and terrestrial ecosystems far from their point of use. In the region of Trier pesticides are widely used. In order to protect crops from pests and increase crop yields in the viniculture, six to eight pesticide applications take place between May and August. The impact that these applications have on the environmental pollution of the region is not yet well understood. The present study was developed to characterize the atmospheric presence, temporal patterns, transport and deposition of a variety of pesticides in the atmosphere of the area of Trier. To this purpose, rain samples were weekly collected at eight sites during the growing seasons 2000, 2001 and 2002, and air samples (gas and particle phases) were collected during the growing season 2002. Multiresidue analysis methods were developed to determine multiple classes of pesticides in rain water, particle- and gas-phase samples. Altogether 24 active ingredients and 3 metabolites were chosen as representative substances, focussing mainly on fungicides. Twenty-four of the 27 measured pesticides were detected in the rain samples; seventeen pesticides were detected in the air samples. The most frequently detected pesticides and at the highest concentrations, both in rain and air, were compounds belonging to the class of fungicides. The insecticide methyl parathion was also detected in several rain samples as well as two substances that are banned in Germany, such as the herbicides atrazine and simazine. Concentration levels varied during the growing season with the highest concentrations being measured in the late spring and summer months, coinciding with application times and warmer months. Concentration levels measured in the rain samples were, generally, in the order of rnng l-1. Though average concentrations for single substances were less than 100 ng l-1, total concentrations were considerable and in some instances well above the EU drinking water quality standard of 500 ng l-1 for total pesticides. Compared to the amounts applied for pest control, the amounts deposited by rain resulted between 0,004% and 0,10% of the maximum application rates. These low pesticide inputs from precipitation to surface-water bodies is not of concern in vinicultural areas where the impact of other sources, such as superficial runoff inputs from the treated areas and cleaning of field crop sprayers, is more important. However, the potential impacts of these aerial pesticide inputs to non-target sites, such as organic crops, and sensitive ecosystems are as yet not known. Concentration levels in the air samples were in the order of ng m-3 at sites close to the fields were pesticides were applied, while lower values, in the order of pg m-3, were detected at the site located further away from fields where applications were performed. The measured air concentration levels found in this study do not represent a concern for human health in terms of acute risk. Inhalation toxicity studies have shown that an acute potential risk only arises at air concentrations in the range of g m-3. Finally, it must be kept in mind that only a small number of chemicals that were applied in the area were analysed for in this study. In order to gain a better evaluation of the local atmospheric load of pesticides, a wider spectrum of applied substances (including metabolites) needs to be investigated.