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A big challenge for agriculture in the 21st century is the provision of food safety to fast growing world- population, which not only demands the well utilisation of the available agricultural resources but also to develop new advancements in the mass production of food crops. Wheat is the third largest food crop of the world and Pakistan is the eighth largest wheat producing country globally. Rice is the second most important staple food of Pakistan after wheat, grown in all provinces of the country. Maize is the world- top ranking food crop followed by wheat and rice. The harvested produts have to be stored in different types of storage structures on small or large scale for food as well as seed purpose. In Pakistan, the harvested grains are stored for the whole year till the introduction of fresh produce in order to ensure the regular food supply throughout the year. However, it is this extended storage period making the commodity more vulnerable to insect attacks. Rhyzopertha dominica (Coleoptera: Bostrychidae), Cryptolestes ferrugineus (Coleoptera: Laemophloeidae), Tribolium castaneum (Coleoptera: Tenebrionidae) and Liposcelis spp. (Psocoptera: Liposcelididae) are the major and most damaging insect pests of stored products all around the world. Various management strategies have been adopted for stored grain insect pests mostly relying upon the use of a broad spectrum of insecticides, but the injudicious use of these chemicals raised various environmental and human health related issues, which necessitate the safe use of the prevailing control measures and evaluation of new and alternative control methods. The application of new chemical insecticides, microbial insecticides (particularly entomopathogenic fungi) and the use of inert dusts (diatomaceous earths) is believed amongst the potential alternatives to generally used insecticides in stored grain insect management system. In the current investigations, laboratory bioassays conducted to evaluate the effects of combining Imidacloprid (new chemistry insecticide) with and without Protect-It (diatomaceous earth formulation) against R. dominica, L. paeta, C. ferrugineus and T. castaneum, on three different grain commodities (i.e. wheat, maize and rice) revealed differences in adult mortality levels among grains and insect species tested. Individually, Imidacloprid was more effective as compared with Protect-It alone and the highest numbers of dead adults were recorded in wheat. The insecticidal efficacy of B. bassiana with Protect-It and DEBBM was also assessed against all test insect species under laboratory conditions. The findings of these studies revealed that the more extended exposure period and the higher combined application rate of B. bassiana and DEs provided the highest mortality of the test insect species. The progeny emergence of each insect species was also greatly suppressed where the highest dose rates of the combined treatments were applied. The residual efficacy of all three control measures Imidacloprid, B. bassiana and DEBBM formulation was also evaluated against all test insect species. The bioassays were carried out after grain treatments and monthly for 6 months. The results indicated that the adult mortality of each test insect species was decreased within the six month storage period, and the integarted application of the test grain protectants enhanced the mortality rates than their alone treatments. The maximum mortality was noted in the combined treatment of DEBBM with Imidacloprid. At the end, the effectiveness of B. bassiana, DEBBM and Imidacloprid applied alone as well as in combinations, against all above mentioned test insect species was also evaluated under field conditions in trials conducted in four districts of Punjab, Pakistan. For each district, a significant difference was observed between treatments, while the combined treatments gave better control of test species as compared with them alone. The least number of surviving adults and minimum percentage of grain damage was observed for the DEBBM and Imidacloprid combination, but DEBBM with B. bassiana provided the best long-term protection as compared with the remaining treatments.
Amphibien sind weltweit stark bedroht, was neben anderen Gründen auch durch Umweltverschmutzung bedingt scheint, wobei hier oft der vermehrte Einsatz von Agrochemikalien hervorgehoben wird. Obwohl landwirtschaftlich genutzte Gebiete meistens stark anthropogen verändert sind, beherbergen sie dennoch viele Amphibienpopulationen. Diese müssen hier oftmals persistieren, weil ihre ursprünglichen Lebensräume für die Landwirtschaft umgestaltet wurden und werden. Neben direkter Habitatzerstörung und "degradierung sowie mechanischen Bearbeitungsprozessen in der Landwirtschaft kann sich der Einsatz von Agrochemikalien, insbesondere von Pestiziden, stark negativ auf die dortigen Amphibien auswirken. Zwar sind aufgrund mangelnder Datenlage bisher keine kausalen Zusammenhänge zwischen Populationsrückgängen und Pestizideinsätzen nachgewiesen, jedoch können Amphibien über verschiedene direkte und indirekte Wege geschädigt werden. Eine Vielzahl von Studien zeigte bereits unterschiedlichste adverse Effekte auf der Individualebene. In der vorliegenden Arbeit wurden wichtige Fragestellungen zu diesem Themengebiet aufgegriffen. Der potenzielle Einfluss von Pestizideinsätzen auf Amphibien, besonders im Freiland, stellt ein komplexes Arbeitsfeld dar, so dass verschiedene methodische Ansätze angewendet wurden, um auf verschiedene Fragestellungen einzugehen. Im ersten Teil der vorliegenden Arbeit wurden zwei methodisch unterschiedliche Risikobewertungen durchgeführt. Es wurde auf die Fragestellung eingegangen, welche Effekte von glyphosatbasierten Herbiziden, welche weltweit den größten Absatz besitzen, auf Amphibien dokumentiert sind und welche Endpunkte am besten geeignet sind, um adverse Effekte nachzuweisen. Dazu wurde eine systematische Literaturübersicht und Meta-Analyse aller publizierten Studien durchgeführt. In einer zweiten Risikobewertung wurde ein GIS-basierter Ansatz angewendet, um das Expositionsrisiko der Amphibienarten des Anhangs II der europäischen Fauna-Flora-Habitat-Richtlinie (Arten von gemeinschaftlichem Interesse) gegenüber Pestiziden in ihren speziell ausgewiesenen Schutzgebieten abzuschätzen. Im zweiten Teil der vorliegenden Arbeit wurden Laborexperimente mit Embryonen und Larven zweier Anuren-Modellorganismen und einem, amphibientoxikologisch bisher noch nicht untersuchten und häufig angewendeten, Herbizid durchgeführt. Der dritte Teil der vorliegenden Arbeit beinhaltet die Ergebnisse zweier Freilandstudien. Es wurde überprüft, ob Anurenlarven aufgrund der Wahrscheinlichkeit der Ausbildung von Deformationen (was im Labor einen gut studierten Endpunkt darstellt) als Bioindikatoren für die Belastung von Kleingewässern mit Agrochemikalien geeignet sind. In der zweiten Freilandstudie wurde überprüft, ob drei einheimische Amphibienarten Kleinstgewässer meiden, welche mit umweltrelevanten Konzentrationen verschiedener Stoffe kontaminiert wurden. Im rechtswissenschaftlichen Teil der Arbeit wurde darauf eingegangen, wie hoch die unterschiedlichen Umsetzungsstandards der europäischen Wasserrahmenrichtlinie respektive des nationalen Wasserhaushaltsgesetzes in den Ländern bezüglich des Schutzes von Kleingewässern, welche fast alle heimischen Amphibienarten zur Reproduktion benötigen, durch Gewässerrandstreifenschutz ist.
Roof and wall slates are fine-grained rocks with slaty cleavage, and it is often difficult to determine their mineral composition. A new norm mineral calculation called slatecalculation allows the determination of a virtual mineral composition based on full chemical analysis, including the amounts of carbon dioxide (CO2), carbon (C), and sulfur (S). Derived norm minerals include feldspars, carbonates, micas, hydro-micas, chlorites, ore-minerals, and quartz. The mineral components of the slate are assessed with superior accuracy compared to the petrographic analysis based on the European Standard EN 12326. The inevitable methodical inaccuracies in the calculations are limited and transparent. In the present paper, slates, shales, and phyllites from worldwide occurrences were examined. This also gives an overview of the rocks used for discontinuous roofing and external cladding.
Considering actual climatic and land use changes the problem of available water resources or the estimation of potential flood risks gain eco-political and economical relevance. Adequate assessments, thus, require precise process-based hydrological knowledge. Spatially distributed hydrological modelling enables a both abstractive and realistic description of hydrological processes, and therefore contributes to the understanding of the hydrological system- responses. Referring to the example of the mesoscale Ruwer basin (a tributary to the Mosel river), a modified version of the distributive modelling system PRMS/MMS (Precipitation Runoff Modeling System/Modular Modeling System) is applied to calculate spatially and temporally explicit water budgets. To achieve modelling results as precise as possible, integration of detailed land use information (spatial distribution of the existing land use classes, crop- and site-specific growth patterns) is necessary. This information is derived here by analysis of multitemporal, geometrically and radiometrically pre-processed Landsat TM-data. This enables separation of different land use classes and differentiated quantification of the leaf area index (LAI). The LAI is estimated by a spectral unmixing approach using statistically optimized endmember sets, referring to the example of winter grain and grassland plots. As a result, numerical inputs (coefficients for calculating evapotranspiration, interception storages) and extracted non-numerical (classified) information can be provided for hydrological modelling. The version of PRMS applied in this study allows important land use terms to be parameterized in high temporal resolution. Using model input derived from the available satellite data, simulation results are obtained that prove to be realistic compared to gauge data and with respect to their spatial differentiation. Results differ significantly from those obtained by using parameters from literature or by experience without distinguishing specific and site-dependent growth patterns. It can be concluded that the quality of modelling results notably improves by integration and quantitative analysis of remote sensing data; thus, these methods are a significant contribution to physically-based hydrological modelling.
Phylogeographic analyses point to long-term survival on the spot in micro-endemic Lycian salamanders
(2020)
Lycian salamanders (genus Lyciasalamandra) constitute an exceptional case of microendemism of an amphibian species on the Asian Minor mainland. These viviparous salamanders are confined to karstic limestone formations along the southern Anatolian coast and some islands. We here study the genetic differentiation within and among 118 populations of all seven Lyciasalamandra species across the entire genus’ distribution. Based on circa 900 base pairs of fragments of the mitochondrial 16SrDNA and ATPase genes, we analysed the spatial haplotype distribution as well as the genetic structure and demographic history of populations. We used 253 geo-referenced populations and CHELSA climate data to infer species distribution models which we projected on climatic conditions of the Last Glacial Maximum (LGM). Within all but one species, distinct phyloclades were identified, which only in parts matched current taxonomy. Most haplotypes (78%) were private to single populations. Sometimes population genetic parameters showed contradicting results, although in several cases they indicated recent population expansion of phyloclades. Climatic suitability of localities currently inhabited by salamanders was significantly lower during the LGM compared to recent climate. All data indicated a strong degree of isolation among Lyciasalamandra populations, even within phyloclades. Given the sometimes high degree of haplotype differentiation between adjacent populations, they must have survived periods of deteriorated climates during the Quaternary on the spot. However, the alternative explanation of male biased dispersal combined with a pronounced female philopatry can only be excluded if independent nuclear data confirm this result.
This paper describes the concept of the hyperspectral Earth-observing thermal infrared (TIR) satellite mission HiTeSEM (High-resolution Temperature and Spectral Emissivity Mapping). The scientific goal is to measure specific key variables from the biosphere, hydrosphere, pedosphere, and geosphere related to two global problems of significant societal relevance: food security and human health. The key variables comprise land and sea surface radiation temperature and emissivity, surface moisture, thermal inertia, evapotranspiration, soil minerals and grain size components, soil organic carbon, plant physiological variables, and heat fluxes. The retrieval of this information requires a TIR imaging system with adequate spatial and spectral resolutions and with day-night following observation capability. Another challenge is the monitoring of temporally high dynamic features like energy fluxes, which require adequate revisit time. The suggested solution is a sensor pointing concept to allow high revisit times for selected target regions (1"5 days at off-nadir). At the same time, global observations in the nadir direction are guaranteed with a lower temporal repeat cycle (>1 month). To account for the demand of a high spatial resolution for complex targets, it is suggested to combine in one optic (1) a hyperspectral TIR system with ~75 bands at 7.2"12.5 -µm (instrument NEDT 0.05 K"0.1 K) and a ground sampling distance (GSD) of 60 m, and (2) a panchromatic high-resolution TIR-imager with two channels (8.0"10.25 -µm and 10.25"12.5 -µm) and a GSD of 20 m. The identified science case requires a good correlation of the instrument orbit with Sentinel-2 (maximum delay of 1"3 days) to combine data from the visible and near infrared (VNIR), the shortwave infrared (SWIR) and TIR spectral regions and to refine parameter retrieval.
Soil loss by water-erosion is world-wide one of the main risks in soil protection. However, the reasons for soil erosion and its degree differ within the different regions of the world. The main focus of the PhD-Theses was to investigate the main factors for soil erosion in two regions differing in parent material, climate, soil type, and land use, namely Rhineland-Palatinate (Germany) and Turkey (Middle Anatolia). The results can be summarized as followed: 1. The salt content and the electric conductivity of the soils in Middle Anatolia were higher compared with the soils in Rhineland-Palatinate. This resulted in a reduced aggregate stability, a reduced water infiltration rate, and consequently in a compaction of the soil surface. 2. The amounts of soil organic matter were lower in the soils of Middle Anatolia compared to the German soils. 3. According to the universal soil loss equation (USLE) the K-factor was higher in the soils of Middle Anatolia compared to the German soils. 4. Just as the K-factor the R-factor was higher in the soils of Middle Anatolia compared to the German soils.
Today obesity has been recognized as a disease. Evidence suggests that obesity often has Genetic, environmental, psychological and other factors. Growing evidence points to heredity as a strong determining factor of obesity. The characterization of uncoupling proteins (UCP) represents a major breakthrough of genetic factors towards understanding the molecular basis for energy expenditure and therefore likely to have important implication for the cause and treatment of human obesity. UCPs as mitochondrial anion carriers which creates a pathway that allows dissipation of the proton electrochemical gradient therefore which when deregulated are key risk factors in the development of obesity and other eating disorders. In order to better understand the roles of both UCP2 and UCP3 which considered as prime candidate genes involved in the pathogenesis of obesity, this study elucidate (1) Genomic organization: The human UCP2 (3) gene spans over 8.7 kb (7.5 kb) distributed on 8 (7) exons. Three UCP genes may have evolved from a common ancestor or are the result from gene duplication events. Two mRNA transcripts are generated from hUCP3 gene, the long and short form of hUCP3 is differing by the presence or absence of 37 amino acid residues at the C-terminus. (2) Mutational analysis revealed a mutation in exon 4 of hUCP2 resulting in the substitution of an alanine by a valine at codon 55 and an insertion polymorphism in exon 8 consisted of a 45 bp repeat located 150 bp downstream of the stop codon in the 3'-UTR. The allele frequencies of both polymorphisms were not significantly elevated in a subgroup of children characterized by low Resting Metabolic Rates (RMR). (3) Promoter Analysis showed that the promoter region of hUCP2 lacks a classical TATA or CAAT box. Functional characterization of hUCP2 promoter showed that minimal promoter activity was observed within 65 bp upstream of the transcriptional start site. 75 bp further upstream a strong cis-acting regulatory element was identified which significantly enhanced basal promoter activity. The regulation of human UCP2 gene expression involves complex interactions among positive and negative regulatory elements. the 5"-flanking region of the hUCP3 gene were characterized in which contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific MyoD and MEF2 sites. Functional characterization identified a cis-acting negative regulatory element between - 2983 and -982 while the region between -982 and -284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.
Investigation of soil water budget in the unsaturated soil zone is commonly performed for determination of the complex relationships between the different components of the water budget. A main focus of this work was on acquisition and evaluation of percolation water and soil moisture dynamics depending on the site properties (climate, soils, land use). A network consisting of 14 lysimeter and soil moisture measuring stations was established in the region of the Trier-Bitburger-Mulde (SW-Eifel, Germany). During a period of four hydrological years precipitation, infiltration, percolation water and soil moisture were collected weakly on the stations comprising the different regional characteristics of soil textures and land use patterns. The analysis of the weekly data sets enabled to point out seasonal variations of percolation water and soil water dynamics (as time-depth function). Comparison of simultaneously collected data sets of percolation water, soil moisture, and evapotranspiration shows significant fluctuations, which result from the complex relationships between the site properties. Hereby, the soil properties and in particular the secondary macropores (root channels, shrinkage- /frost cracks) have a striking influence on the variability of the water flows in the unsaturated soil zone. The results of the GIS-based regionalisation of the annual amount of percolation water prove the influence of topography (relief conditions, especially slope and aspect) on percolation and soil water dynamics in low mountain ranges.
The skin is continuously challenged by environmental antigens that may penetrate and elicit a skin sensitization, which can develop into allergic contact dermatitis. Medical treatment for allergic contact dermatitis is limited - in fact only acute symptoms can be cured and for secondary prevention of the disease a lifelong avoidance of the allergen(s) is necessary. Therefore, the screening of the sensitization potential of substance used in commercially available products is indispensable to prevent such diseases. Hence, risk assessment is deduced from data obtained by murine local lymph node assay predominantly, but there exists a need to develop methods capable of providing the same information that do not require the use of animals in view of legislative initiatives such as REACH (registration, evaluation, authorization of chemicals) as well as the 7th Amendment to the Cosmetics Directive (2003/15/EC). Therefore, a number of promising in silico and in vitro approaches are being developed to address this need. In vitro test systems using the response of dendritic cells, which are the key player in the elicitation process of contact dermatitis, are established, but, although these novel methods for hazard identification might find application in the context of screening, it is not clear whether these approaches are useful for the purposes of risk assessment and risk management to predict allergic potency. Therefore, it was investigated whether on the one hand in vitro generated dendritic cells from primary blood monocytes (MoDC) and on the other hand a continuous monocytic cell line, the THP-1 cells, suggested as dendritic cell surrogate, react to a presumably weak allergen. Ascaridol, predicted as one of the possible causes for tea tree oil contact dermatitis, was studied and its effects in these two in vitro skin sensitization models were explored. Thus, the surface expression of CD86, HLADR, CD54, and CD40, which are known as activation markers in both in vitro models, were measured via flow cytometry. For MoDC, an augmented CD86 and HLADR surface expression in comparison to untreated cells were determined after 24 h exposure with ascaridol. An increased CD54 and CD40 surface expression were found only in some donors. After long term incubation of 96 h, ascaridol-treated MoDC still up-regulated CD86 and additionally an augmented CD40 expression was measured in all studied donors. An enhanced CD54 expression was determined for 50 percentage of all investigated donors. Furthermore, CD80, CD83 and CD209 protein expression were up-regulated in MoDC after 96 h of ascaridol incubation. In addition, it was determined that after 24 h ascaridol-treated MoDC showed an increased capacity to uptake antigens, whereas after 96 h this capacity got lost and antigen-capturing devices were reduced in comparison to non-treated MoDC. Moreover, the cytokine release of ascaridol-treated MoDC were measured after 24 h. Tumor necrosis factor (TNF)alpha, interleukin (IL)-1beta and IL 6 secretion were determined in some donors. Furthermore, IL-8 release was clearly increased after 24 h ascaridol treatment. By the same token, THP-1 cells were analyzed after ascaridol treatment for several activation markers. We found a similar response pattern as measured in MoDC. Ascaridol induced CD86 expression as well as CD54 after 24 h incubation. Additionally, the impact of ascaridol on phosphorylation of p38 mitogen-activated protein kinase, which had been shown to be involved in increased expression of activation markers like CD86 by others, were studied via Western blot analysis. A phosphorylation of p38 was determined after 15 min of ascaridol stimulation. Moreover, an augmented CD40 and HLADR surface expression were measured in a dose-response manner after 24 h ascaridol treatment. Also similar to MoDC an enhanced IL-8 secretion after ascaridol stimulation was observed in THP-1 cells. Hence, for the first time it was shown that ascaridol has immuno-modulating effects. The obtained data from both in vitro systems, MoDC and THP-1 cells, identified ascaridol as a sensitizer. Although for both systems there remain significant challenges to overcome for potency assessment, ascaridol is presumed to be a weak sensitizer probably. Interestingly, ascaridol treatment of THP-1 cells resulted also in an increased augmentation of CD184 and CCR2, two chemokine receptors expressed on monocyte. Therefore, these data encouraged the exploration of chemokine receptors as tools in skin sensitization prediction. Consequently, the combination of chemical assays with in vitro techniques may provide a useful surrogate to animal testing for skin sensitization. Due to the continuously changing environmental conditions, it is necessary to regularly monitor and update the spectrum of sensitizers that elicit contact dermatitis. Therefore, both debated in vitro test systems will become indispensable tools.